PMID- 35357625 OWN - NLM STAT- MEDLINE DCOM- 20220713 LR - 20220715 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 49 IP - 7 DP - 2022 Jul TI - Ultrasound-targeted microbubble destruction (UTMD)-mediated miR-150-5p attenuates oxygen and glucose deprivation-induced cardiomyocyte injury by inhibiting TTC5 expression. PG - 6041-6052 LID - 10.1007/s11033-022-07392-3 [doi] AB - BACKGROUND: Cardiomyocyte injury is a typical feature in cardiovascular diseases. Changes in cardiomyocytes strongly affect the progression of cardiovascular diseases. This work aimed to investigate the biological function and potential mechanism of action of miR-150-5p in cardiomyocytes. METHODS AND RESULTS: A myocardial ischemia (MI) injury rat model was constructed to detect miR-150-5p and tetratricopeptide repeat domain 5 (TTC5) expression during heart ischemia injury. Primary cardiomyocytes were isolated for in vitro study. CCK-8 assays were used to detect cardiomyocyte viability. Western blots were used to detect TTC5 and P53 expression. qPCR was utilized to measure RNA expression of miR-150-5p and TTC5. The TUNEL assay was used to determine cell apoptosis. ELISA was used to determine cytokine (TNF-alpha, IL-1beta, IL-6, and IL-8) levels in heart tissues and cell culture supernatants. A dual-luciferase reporter assay was carried out to verify the binding ability between miR-150-5p and TTC5. Oxygen-glucose deprivation (OGD) treatment significantly inhibited cell viability. Ultrasound-targeted microbubble destruction (UTMD)-mediated uptake of miR-150-5p inverted these results. Additionally, UTMD-mediated uptake of miR-150-5p retarded the effects of OGD treatment on cell apoptosis. Besides, UTMD-mediated uptake of miR-150-5p counteracted the effects of OGD treatment on the inflammatory response by regulating cytokine (TNF-alpha, IL-1beta, IL-6, and IL-8) levels. For the mechanism of the protective effect on the heart, we predicted and confirmed that miR-150-5p bound to TTC5 and inhibited TTC5 expression. CONCLUSIONS: UTMD-mediated uptake of miR-150-5p attenuated OGD-induced primary cardiomyocyte injury by inhibiting TTC5 expression. This discovery contributes toward further understanding the progression of primary cardiomyocyte injury. CI - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Zhong, Xin AU - Zhong X AD - Department of Ultrasound, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410002, Hunan, China. FAU - Chen, Yu AU - Chen Y AD - Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China. FAU - Long, Xiangdang AU - Long X AD - Department of Ultrasound, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410002, Hunan, China. FAU - Chen, Hongtian AU - Chen H AD - Department of Ultrasound, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410002, Hunan, China. FAU - Zheng, Zhaofen AU - Zheng Z AD - Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China. FAU - Pan, Hongwei AU - Pan H AD - Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China. FAU - Peng, Jianqiang AU - Peng J AD - Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China. FAU - Liu, Yanfu AU - Liu Y AD - Department of Cardiovascular, Lixian People's Hospital, Changde, 415500, Hunan, China. FAU - Wang, Haijun AU - Wang H AD - Department of Cardiovascular, Lixian People's Hospital, Changde, 415500, Hunan, China. FAU - Hu, Yongjun AU - Hu Y AD - Department of Cardiovascular, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, 61, Jiefang West Road, Furong District, Changsha City, 410002, Hunan, China. kuqsdi@163.com. LA - eng GR - No. 2019JJ80080/natural science foundation of hunan province/ GR - No. B2019062/hunan provincial health commission/ GR - No. 20200198/hunan provincial health commission/ PT - Journal Article DEP - 20220331 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (Interleukin-6) RN - 0 (Interleukin-8) RN - 0 (MIRN150 microRNA, rat) RN - 0 (MicroRNAs) RN - 0 (Transcription Factors) RN - 0 (Tumor Necrosis Factor-alpha) RN - IY9XDZ35W2 (Glucose) RN - S88TT14065 (Oxygen) SB - IM MH - Animals MH - Apoptosis MH - *Brain Ischemia/metabolism MH - Glucose/metabolism MH - Interleukin-6/metabolism MH - Interleukin-8/pharmacology MH - *MicroRNAs/metabolism MH - Microbubbles MH - Myocytes, Cardiac/metabolism MH - Oxygen/metabolism MH - Rats MH - Transcription Factors/*metabolism MH - Tumor Necrosis Factor-alpha/metabolism OTO - NOTNLM OT - Cell viability OT - Primary cardiomyocyte injury OT - Progression OT - TTC5 OT - UTMD OT - miR-150-5p EDAT- 2022/04/01 06:00 MHDA- 2022/07/14 06:00 CRDT- 2022/03/31 12:06 PHST- 2021/07/26 00:00 [received] PHST- 2022/03/16 00:00 [accepted] PHST- 2022/04/01 06:00 [pubmed] PHST- 2022/07/14 06:00 [medline] PHST- 2022/03/31 12:06 [entrez] AID - 10.1007/s11033-022-07392-3 [pii] AID - 10.1007/s11033-022-07392-3 [doi] PST - ppublish SO - Mol Biol Rep. 2022 Jul;49(7):6041-6052. doi: 10.1007/s11033-022-07392-3. Epub 2022 Mar 31.