PMID- 35358839
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220512
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 606
DP  - 2022 May 28
TI  - Species-independent stimulation of osteogenic differentiation induced by 
      osteoclasts.
PG  - 149-155
LID - S0006-291X(22)00460-0 [pii]
LID - 10.1016/j.bbrc.2022.03.115 [doi]
AB  - The coupling of bone resorption and bone formation is well-recognized in the bone 
      remodeling process, in which osteoblasts and osteoclasts are key players. 
      However, the anabolic effect of human primary osteoclasts has rarely been 
      reported as mouse and cell line derived osteoclasts were mostly used in previous 
      reports. Therefore, a comprehensive comparison of mouse and human osteoclasts and 
      their corresponding functions is needed to study cell-cell interactions between 
      osteoclasts and osteoblasts. Osteoclasts from mouse and human origin were 
      generated, characterized and compared, after which their anabolic effects on the 
      osteogenic differentiation of mouse and human MSCs were assessed. Both murine 
      RAW264.7 derived osteoclasts (mOCs) and primary human osteoclasts (hOCs) derived 
      from buffy coats characteristically displayed multinuclearity, marked integrin beta3 
      expression and enhanced TRAP activity. Despite comparable cell size, mOCs showed 
      higher osteoclast density (number of osteoclasts per cm(2) culture dish) and 
      osteoclast nuclearity (average number of nuclei per osteoclast), but lower TRAP 
      activity compared to hOCs. Culturing primary rat and human bone marrow MSCs with 
      the conditioned medium of mOCs or hOCs showed anabolic effects regarding the 
      osteogenic differentiation of MSCs with superiority of hOCs over mOCs. We 
      conclude that despite morphological and functional differences between mouse and 
      human osteoclasts, their secretory factors evoke similar anabolic effects on MSC 
      osteogenic differentiation.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - School of Stomatology, Health Science Center, Shenzhen University, Shenzhen, 
      518000, China; Department of Dentistry - Regenerative Biomaterials, Radboudumc, 
      Nijmegen, 6525EX, the Netherlands.
FAU - Polman, Marjolein
AU  - Polman M
AD  - Department of Dentistry - Regenerative Biomaterials, Radboudumc, Nijmegen, 
      6525EX, the Netherlands.
FAU - Mohammad, Abdullah Faqeer
AU  - Mohammad AF
AD  - School of Stomatology, Health Science Center, Shenzhen University, Shenzhen, 
      518000, China.
FAU - Hermens, Inge
AU  - Hermens I
AD  - Department of Dentistry - Regenerative Biomaterials, Radboudumc, Nijmegen, 
      6525EX, the Netherlands.
FAU - Zhuang, Zhumei
AU  - Zhuang Z
AD  - School of Bioengineering, Dalian University of Technology, Dalian, 116023, China.
FAU - Wang, Huanan
AU  - Wang H
AD  - School of Bioengineering, Dalian University of Technology, Dalian, 116023, China.
FAU - van den Beucken, Jeroen Jjp
AU  - van den Beucken JJ
AD  - Department of Dentistry - Regenerative Biomaterials, Radboudumc, Nijmegen, 
      6525EX, the Netherlands; Radboud Institute for Molecular Life Sciences (RIMLS) - 
      theme Reconstructive & Regenerative Medicine, Nijmegen, 6500HB, the Netherlands. 
      Electronic address: jeroen.vandenbeucken@radboudumc.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220325
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anabolic Agents)
SB  - IM
MH  - *Anabolic Agents/metabolism/pharmacology
MH  - Animals
MH  - *Bone Resorption/metabolism
MH  - Cell Differentiation
MH  - Mice
MH  - Osteoblasts/metabolism
MH  - Osteoclasts/metabolism
MH  - Osteogenesis
MH  - Rats
OTO - NOTNLM
OT  - Anabolic effects
OT  - Co-culture
OT  - MSCs
OT  - Osteoclasts
OT  - Species difference
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/04/01 06:00
MHDA- 2022/04/27 06:00
CRDT- 2022/03/31 20:14
PHST- 2022/02/21 00:00 [received]
PHST- 2022/03/21 00:00 [revised]
PHST- 2022/03/22 00:00 [accepted]
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/03/31 20:14 [entrez]
AID - S0006-291X(22)00460-0 [pii]
AID - 10.1016/j.bbrc.2022.03.115 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 May 28;606:149-155. doi: 
      10.1016/j.bbrc.2022.03.115. Epub 2022 Mar 25.