PMID- 35359323
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20221221
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2450
DP  - 2022
TI  - Monitoring Telomere Maintenance During Regeneration of Annelids.
PG  - 467-478
LID - 10.1007/978-1-0716-2172-1_24 [doi]
AB  - Telomere shortening is a hallmark of aging and eventually constrains the 
      proliferative capacity of cells. The protocols discussed here are used for 
      monitoring telomeres comprehensively in Aeolosoma viride, a model system for 
      regeneration studies. We present methods for analyzing the activity of telomerase 
      enzyme in regenerating tissue by telomeric repeat amplification protocol (TRAP) 
      assay, for comparing telomere length between existing tissue and newly 
      regenerated tissue by telomere restriction fragment (TRF) assay, as well as for 
      visualizing telomeres by fluorescence in situ hybridization (FISH).
CI  - (c) 2022. The Author(s).
FAU - Joseph, Nithila A
AU  - Joseph NA
AD  - Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
FAU - Chen, Chi-Fan
AU  - Chen CF
AD  - Department of Life Sciences, National Taiwan University, Taipei, Taiwan.
FAU - Chen, Jiun-Hong
AU  - Chen JH
AD  - Department of Life Sciences, National Taiwan University, Taipei, Taiwan. 
      chenjh@ntu.edu.tw.
FAU - Chen, Liuh-Yow
AU  - Chen LY
AD  - Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan. 
      lyowchen@gate.sinica.edu.tw.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - *Annelida
MH  - In Situ Hybridization, Fluorescence
MH  - *Telomerase/genetics/metabolism
MH  - Telomere/genetics/metabolism
MH  - Telomere Homeostasis
PMC - PMC9761496
OTO - NOTNLM
OT  - Aeolosoma viride
OT  - Regeneration
OT  - TRAP assay
OT  - TRF assay
OT  - Telomere FISH
OT  - Telomere maintenance
EDAT- 2022/04/02 06:00
MHDA- 2022/04/05 06:00
PMCR- 2022/12/19
CRDT- 2022/04/01 05:08
PHST- 2022/04/01 05:08 [entrez]
PHST- 2022/04/02 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/12/19 00:00 [pmc-release]
AID - 10.1007/978-1-0716-2172-1_24 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2450:467-478. doi: 10.1007/978-1-0716-2172-1_24.