PMID- 3536318
OWN - NLM
STAT- MEDLINE
DCOM- 19870116
LR  - 20190828
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 5
IP  - 11
DP  - 1986 Nov
TI  - Investigation of sloughed corneal epithelial cells collected by non-invasive 
      irrigation of the corneal surface.
PG  - 847-56
AB  - This paper reports the development of a non-contact corneal irrigation chamber 
      (NC-CIC) which enables non-invasive collection of epithelial cells from the 
      corneal surface of human subjects. Cells were viewed by fluorescence microscopy 
      following vital staining with acridine orange (AO). Staining characteristics 
      revealed two corneal epithelial cell types: cells with (i) green and (ii) 
      orange-red cytoplasmic staining. The green cytoplasmic stain appeared to indicate 
      a more viable cell. Multi-cell aggregates were regularly collected from the 
      corneal epithelial surface. Groups of up to seven epithelial cells were obtained. 
      Quantitative studies of corneal epithelial cell sloughing, using isotonic NaCl 
      (305 mOsm/kg) and isotonic "basic tear solution" (BTS, 305 mOsm/kg) as irrigating 
      solutions, involving hourly irrigations between 8 a.m. and 4 p.m. were conducted. 
      Consistently higher cell counts were obtained with NaCl. Using BTS, data scatter 
      was reduced sufficiently to reveal significant differences in sloughing rate as a 
      function of time of day. Instillation of one drop of 0.5% proparacaine caused a 
      significant, but gradual, increase in epithelial cell sloughing rate over a 
      period of hours, as indicated by subsequent BTS irrigations of the cornea. Since 
      the NC-CIC technique is able to discriminate these effects, it may be an 
      appropriate system for in vivo studies of the relationship between corneal 
      epithelial cell mitosis and sloughing.
FAU - Fullard, R J
AU  - Fullard RJ
FAU - Wilson, G S
AU  - Wilson GS
LA  - eng
GR  - EY03039/EY/NEI NIH HHS/United States
GR  - RR05807/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - B4OB0JHI1X (proxymetacaine)
RN  - EPD1EH7F53 (Propoxycaine)
SB  - IM
MH  - Anesthesia, Local
MH  - Cell Count
MH  - Conjunctiva/cytology
MH  - Cornea/*cytology
MH  - *Cytological Techniques
MH  - Epithelial Cells
MH  - Humans
MH  - Propoxycaine
MH  - Specimen Handling/*methods
MH  - Therapeutic Irrigation/instrumentation
EDAT- 1986/11/01 00:00
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PHST- 1986/11/01 00:00 [pubmed]
PHST- 1986/11/01 00:01 [medline]
PHST- 1986/11/01 00:00 [entrez]
AID - 10.3109/02713688609029236 [doi]
PST - ppublish
SO  - Curr Eye Res. 1986 Nov;5(11):847-56. doi: 10.3109/02713688609029236.