PMID- 35364708 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220421 IS - 2192-2195 (Print) IS - 2192-2209 (Electronic) IS - 2192-2209 (Linking) VI - 12 IP - 1 DP - 2022 Apr 2 TI - Indole alkaloids of Alstonia scholaris (L.) R. Br. alleviated nonalcoholic fatty liver disease in mice fed with high-fat diet. PG - 14 LID - 10.1007/s13659-022-00335-2 [doi] LID - 14 AB - Alstonia scholaris (L.) R. Br (Apocynaceae) is a well-documented medicinal plant for treating respiratory diseases, liver diseases and diabetes traditionally. The current study aimed to investigate the effects of TA on non-alcoholic fatty liver disease (NAFLD). A NAFLD model was established using mice fed a high-fat diet (HFD) and administered with TA (7.5, 15 and 30 mg/kg) orally for 6 weeks. The biochemical parameters, expressions of lipid metabolism-related genes or proteins were analyzed. Furthermore, histopathological examinations were evaluated with Hematoxylin-Eosin and MASSON staining. TA treatment significantly decreased the bodyweight of HFD mice. The concentrations of low-density lipoprotein (LDL), triglyceride (TG), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also decreased significantly in TA-treated mice group, accompanied by an increase in high-density lipoprotein (HDL). Furthermore, TA alleviated hepatic steatosis injury and lipid droplet accumulation of liver tissues. The liver mRNA levels involved in hepatic lipid synthesis such as sterol regulatory element-binding protein 1C (SREBP-1C), regulators of liver X receptor alpha (LXRalpha), peroxisome proliferator activated receptor (PPAR)gamma, acetyl-CoA carboxylase (ACC1) and stearyl coenzyme A dehydrogenase-1 (SCD1), were markedly decreased, while the expressions involved in the regulation of fatty acid oxidation, PPARalpha, carnitine palmitoyl transterase 1 (CPT1A), and acyl coenzyme A oxidase 1 (ACOX1) were increased in TA-treated mice. TA might attenuate NAFLD by regulating hepatic lipogenesis and fatty acid oxidation. CI - (c) 2022. The Author(s). FAU - Sun, Shui-Fen AU - Sun SF AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. AD - School of Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. FAU - Zhong, Hui-Jie AU - Zhong HJ AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. AD - School of Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. AD - Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. FAU - Zhao, Yun-Li AU - Zhao YL AD - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China. FAU - Ma, Xiu-Ying AU - Ma XY AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. FAU - Luo, Jin-Bo AU - Luo JB AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. FAU - Zhu, Ling AU - Zhu L AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. FAU - Zhang, Yu-Ting AU - Zhang YT AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. FAU - Wang, Wen-Xue AU - Wang WX AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. wenxue_wang@163.com. AD - School of Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. wenxue_wang@163.com. FAU - Luo, Xiao-Dong AU - Luo XD AUID- ORCID: 0000-0002-6768-5679 AD - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China. xdluo@mail.kib.ac.cn. FAU - Geng, Jia-Wei AU - Geng JW AD - Department of Infectious Disease and Hepatic Disease, First People's Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650032, Yunnan, China. Jiawei_Geng@kmust.edu.cn. AD - School of Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. Jiawei_Geng@kmust.edu.cn. AD - Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China. Jiawei_Geng@kmust.edu.cn. LA - eng GR - 81860437/National Natural Science Foundation of China/ GR - YNWR-MY-2019-072/Prominent Physician Project of Yunnan province/ GR - 2019ZF004/Yunnan Major Science and Technology Project/ GR - 2019LCZXKF-XH05/Digestive Endoscopy Medical Center/ GR - 202005AE160010/Yunnan Province Innovation Team of Intestinal Microecology-Related Disease Research and Technological Transformation/ PT - Journal Article DEP - 20220402 PL - Germany TA - Nat Prod Bioprospect JT - Natural products and bioprospecting JID - 101577734 PMC - PMC8975985 OTO - NOTNLM OT - Fatty acid oxidation OT - Hepatic disease OT - Hepatic lipogenesis COIS- No potential conflict of interest was reported by the author(s). EDAT- 2022/04/03 06:00 MHDA- 2022/04/03 06:01 PMCR- 2022/04/02 CRDT- 2022/04/02 05:04 PHST- 2022/02/17 00:00 [received] PHST- 2022/03/17 00:00 [accepted] PHST- 2022/04/02 05:04 [entrez] PHST- 2022/04/03 06:00 [pubmed] PHST- 2022/04/03 06:01 [medline] PHST- 2022/04/02 00:00 [pmc-release] AID - 10.1007/s13659-022-00335-2 [pii] AID - 335 [pii] AID - 10.1007/s13659-022-00335-2 [doi] PST - epublish SO - Nat Prod Bioprospect. 2022 Apr 2;12(1):14. doi: 10.1007/s13659-022-00335-2.