PMID- 35367779
OWN - NLM
STAT- MEDLINE
DCOM- 20220419
LR  - 20220419
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 185
DP  - 2022 Jun
TI  - Porcine reproductive and respiratory syndrome virus induces tight junction 
      barrier dysfunction and cell death in porcine glandular endometrial epithelial 
      cells.
PG  - 34-42
LID - S0093-691X(22)00110-8 [pii]
LID - 10.1016/j.theriogenology.2022.03.021 [doi]
AB  - Reproductive failure caused by porcine reproductive and respiratory syndrome 
      virus (PRRSV) is characterized by embryonic death and weak-born piglets and is 
      associated with placental cell apoptosis and impairment of endometrial integrity. 
      Here, we aimed to determine whether endometrial epithelial barrier function and 
      viability were altered following PRRSV type 1 or type 2 infection. PRRSV 
      inoculation was examined at the apical or basolateral side of porcine glandular 
      endometrial epithelial cell cultures isolated from 4- to 6-month-old PRRSV-free 
      herd gilts (n = 7 pigs). On the apical side, four days postinfection (4 dpi) with 
      type 2 PRRSV, transepithelial electrical resistance decreased by 31% +/- 5%, and 
      paracellular permeability to fluorescein isothiocyanate-dextran (4 kDa) increased 
      by 10-fold as compared with the mock and type 1 infection. Real-time polymerase 
      chain reaction results revealed that both PRRSV types upregulated the mRNA 
      expression of the barrier builder tight junction protein (TJ) Cldn5, but 
      downregulated pore-forming TJ Cldn7. Additionally, the expression of other TJ 
      genes, i.e., Cldn3 and Cldn8, was differentially increased by PRRSV type 1 and 
      that of zonula occludens-1 was increased by PRRSV type 2. MTT assays indicated an 
      increase in porcine glandular endometrial epithelial cell culture at 2-6 dpi 
      following type 2 infection. Analysis of apoptosis using Annexin/propidium iodide 
      staining combined with flow cytometry showed that the percentage of viable cells 
      decreased, accompanied by a significantly higher dead cell population following 
      PRRSV type 2 infection at 2-4 dpi. PRRSV type 1 infection also induced dead cells 
      (>4%) at 2 dpi; however, the cell population recovered at 4 dpi. In conclusion, 
      PRRSV type 2 infection caused more severe TJ barrier dysfunction and reduced cell 
      viability compared with PRRSV type 1 infection in the porcine endometrium. 
      Impairment in the membrane integrity of the maternal glandular endometrium may be 
      the underlying mechanism of PRRSV-induced reproductive failure in pregnant sows.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Rukarcheep, Dran
AU  - Rukarcheep D
AD  - Department of Physiology, Faculty of Veterinary Science, Chulalongkorn 
      University, Henri-Dunant Rd., Pathum Wan, Bangkok, 10330, Thailand.
FAU - Lothong, Muttarin
AU  - Lothong M
AD  - Department of Physiology, Faculty of Veterinary Science, Chulalongkorn 
      University, Henri-Dunant Rd., Pathum Wan, Bangkok, 10330, Thailand.
FAU - Wattanaphansak, Suphot
AU  - Wattanaphansak S
AD  - Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn 
      University, Henri-Dunant Rd., Pathum Wan, Bangkok, 10330, Thailand.
FAU - Deachapunya, Chatsri
AU  - Deachapunya C
AD  - Department of Physiology, Faculty of Medicine, Srinakharinwirot University, 
      Sukhumvit Rd., Wattana, Bangkok, 10110, Thailand.
FAU - Poonyachoti, Sutthasinee
AU  - Poonyachoti S
AD  - Department of Physiology, Faculty of Veterinary Science, Chulalongkorn 
      University, Henri-Dunant Rd., Pathum Wan, Bangkok, 10330, Thailand; CU-Animal 
      Fertility Research Unit, Chulalongkorn University, Henri-Dunant Rd., Pathum Wan, 
      Bangkok, 10330, Thailand. Electronic address: Sutthasinee.p@chula.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20220325
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Endometrium/metabolism
MH  - Epithelial Cells
MH  - Female
MH  - Placenta
MH  - *Porcine Reproductive and Respiratory Syndrome/metabolism
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Pregnancy
MH  - Sus scrofa
MH  - Swine
MH  - *Swine Diseases/metabolism
MH  - Tight Junctions
OTO - NOTNLM
OT  - Apoptosis
OT  - Claudin
OT  - Epithelial barrier function
OT  - Glandular endometrium
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2022/04/04 06:00
MHDA- 2022/04/20 06:00
CRDT- 2022/04/03 20:28
PHST- 2021/10/08 00:00 [received]
PHST- 2022/03/18 00:00 [revised]
PHST- 2022/03/21 00:00 [accepted]
PHST- 2022/04/04 06:00 [pubmed]
PHST- 2022/04/20 06:00 [medline]
PHST- 2022/04/03 20:28 [entrez]
AID - S0093-691X(22)00110-8 [pii]
AID - 10.1016/j.theriogenology.2022.03.021 [doi]
PST - ppublish
SO  - Theriogenology. 2022 Jun;185:34-42. doi: 10.1016/j.theriogenology.2022.03.021. 
      Epub 2022 Mar 25.