PMID- 35368277
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220405
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 16
DP  - 2022
TI  - Sympathetic Pathways Target Cholinergic Neurons in the Human Colonic Myenteric 
      Plexus.
PG  - 863662
LID - 10.3389/fnins.2022.863662 [doi]
LID - 863662
AB  - BACKGROUND: The sympathetic nervous system inhibits human colonic motility 
      largely by effects on enteric neurons. Noradrenergic axons, which branch 
      extensively in the myenteric plexus, are integral to this modulatory role, but 
      whether they contact specific types of enteric neurons is unknown. The purpose of 
      this study was to determine the association of noradrenergic varicosities with 
      types of enteric neurons. METHODS: Human colonic tissue from seven patients was 
      fixed and dissected prior to multi-layer immunohistochemistry for human RNA 
      binding proteins C and D (HuC/D) (pan-neuronal cell body labelling), tyrosine 
      hydroxylase (TH, catecholaminergic labelling), Enkephalin (ENK), choline 
      acetyltransferase (ChAT, cholinergic labelling) and/or nitric oxide synthase 
      (NOS, nitrergic labelling) and imaged using confocal microscopy. 
      TH-immunoreactive varicose nerve endings and myenteric cell bodies were 
      reconstructed as three dimensional digital images. Data was exported to a 
      purpose-built software package which quantified the density of varicosities close 
      to the surface of each myenteric cell body. RESULTS: TH-immunoreactive 
      varicosities had a greater mean density within 1 mum of the surface of ChAT +/NOS- 
      nerve cell bodies compared with ChAT-/NOS + cell bodies. Similarly, 
      ENK-immunoreactive varicosities also had a greater mean density close to ChAT 
      +/NOS- cell bodies compared with ChAT-/NOS + cells. CONCLUSION: A method for 
      quantifying close associations between varicosities and nerve cell bodies was 
      developed. Sympathetic axons in the myenteric plexus preferentially target 
      cholinergic excitatory cells compared to nitrergic neurons (which are largely 
      inhibitory). This connectivity is likely to be involved in inhibitory modulation 
      of human colonic motility by the sympathetic nervous system.
CI  - Copyright (c) 2022 Parker, Wiklendt, Humenick, Chen, Sia, Wattchow, Dinning and 
      Brookes.
FAU - Parker, Dominic R
AU  - Parker DR
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
AD  - Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford 
      Park, SA, Australia.
FAU - Wiklendt, Lukasz
AU  - Wiklendt L
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
FAU - Humenick, Adam
AU  - Humenick A
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
FAU - Chen, Bao Nan
AU  - Chen BN
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
FAU - Sia, Tiong Cheng
AU  - Sia TC
AD  - Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford 
      Park, SA, Australia.
FAU - Wattchow, David A
AU  - Wattchow DA
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
AD  - Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford 
      Park, SA, Australia.
FAU - Dinning, Phil G
AU  - Dinning PG
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
FAU - Brookes, Simon J H
AU  - Brookes SJH
AD  - Laboratory of Neurogastroenterology, College of Medicine and Public Health, 
      Flinders University, Adelaide, SA, Australia.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC8970288
OTO - NOTNLM
OT  - colon
OT  - enteric nervous system
OT  - gastrointestinal motility
OT  - human
OT  - myenteric plexus
OT  - sympathetic nervous system
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/05 06:00
MHDA- 2022/04/05 06:01
PMCR- 2022/01/01
CRDT- 2022/04/04 05:06
PHST- 2022/01/27 00:00 [received]
PHST- 2022/02/21 00:00 [accepted]
PHST- 2022/04/04 05:06 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/05 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2022.863662 [doi]
PST - epublish
SO  - Front Neurosci. 2022 Mar 17;16:863662. doi: 10.3389/fnins.2022.863662. 
      eCollection 2022.