PMID- 35369479
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220405
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - The Development of a Real-Time Recombinase-Aid Amplification Assay for Rapid 
      Detection of African Swine Fever Virus.
PG  - 846770
LID - 10.3389/fmicb.2022.846770 [doi]
LID - 846770
AB  - African swine fever (ASF), caused by the African swine fever virus (ASFV), is an 
      acute, deadly, infectious disease of domestic pigs and wild boars and has a 
      tremendous negative socioeconomic impact on the swine industry. ASF is a 
      notifiable disease to the World Organization for Animal Health (OIE). Currently, 
      no effective vaccine or treatment against ASF is available. Early detection and 
      rapid diagnosis are potentially significant to control ASF spread with the 
      emerging ASFV mutant strains and non-classical symptoms. In this study, we 
      developed a real-time recombinase-aid amplification (RAA) assay to detect the 
      ASFV genome rapidly. Thirty samples were detected using commercial lysis buffer 
      for DNA extraction and equipped with a portable testing instrument. The results 
      showed that the sensitivity of RAA was 10(3) copies per reaction at 95% 
      probability in 9 min at 39 degrees C. The method was universally specific for three 
      strains of ASFV, and there was no cross-reaction with other pathogens, including 
      foot-and-mouth disease virus (FMDV), classical swine fever virus (CSFV), porcine 
      reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 (PCV2), 
      pseudorabies virus (PRV), and porcine parvovirus (PPV). The coefficient of 
      variation (C.V) of repetitive experiments was 0%, and the coincidence rate was 
      100% compared to the real-time qPCR. 123 field samples were detected by the 
      real-time RAA assay, and the results showed that the clinical coincidence rate of 
      the real-time RAA assay was 98% compared to the real-time qPCR assay. The 
      advantages of this method were as follows: the extraction of DNA can be performed 
      on site, the DNA template is directly used, a small battery-powered instrument is 
      easily available, and the on-site diagnostic process is finished within an hour. 
      These suggest that this assay could be used to detect different genotypes of ASFV 
      and play a vital role in the control of ASF.
CI  - Copyright (c) 2022 Wu, Yang, Ru, Qin, Li, Zhang, Zhang, Li, Zhang and Li.
FAU - Wu, Yongshu
AU  - Wu Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Ru, Yi
AU  - Ru Y
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Qin, Xiaodong
AU  - Qin X
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Li, Miaomiao
AU  - Li M
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Zhang, Zhixiong
AU  - Zhang Z
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - College of Animal Science and Veterinary Medicine, Southwest Minzu University, 
      Chengdu, China.
FAU - Li, Yijing
AU  - Li Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Zhang, Zhidong
AU  - Zhang Z
AD  - College of Animal Science and Veterinary Medicine, Southwest Minzu University, 
      Chengdu, China.
FAU - Li, Yanmin
AU  - Li Y
AD  - College of Animal Science and Veterinary Medicine, Southwest Minzu University, 
      Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC8969597
OTO - NOTNLM
OT  - African swine fever virus
OT  - nucleic acid detection
OT  - portable instrument
OT  - qPCR
OT  - real-time recombinase-aid amplification assay
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/05 06:00
MHDA- 2022/04/05 06:01
PMCR- 2022/03/17
CRDT- 2022/04/04 05:20
PHST- 2021/12/31 00:00 [received]
PHST- 2022/01/24 00:00 [accepted]
PHST- 2022/04/04 05:20 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/05 06:01 [medline]
PHST- 2022/03/17 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.846770 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Mar 17;13:846770. doi: 10.3389/fmicb.2022.846770. 
      eCollection 2022.