PMID- 3537015
OWN - NLM
STAT- MEDLINE
DCOM- 19870121
LR  - 20190828
IS  - 0303-6979 (Print)
IS  - 0303-6979 (Linking)
VI  - 13
IP  - 9
DP  - 1986 Oct
TI  - Blastogenetic responsiveness of peripheral blood lymphocytes from patients with 
      adult periodontitis, evaluated by a reverse hemolytic plaque assay.
PG  - 805-9
AB  - Peripheral blood lymphocytes were obtained from 10 patients with advanced 
      periodontitis and from 5 normal control subjects. The lymphocytes were cultured 
      with dental plaque, supernatant of homologous fibroblast cytoplasm and 
      Pokeweed-mitogen (PWM). The number of immunoglobulin-secreting cells (ISC) was 
      assessed after 7 days using a reverse hemolytic plaque assay (RHPA). The 
      stimulation levels of lymphocytes from periodontitis patients and control 
      subjects stimulated with dental plaque and PWM were not statistically different 
      (p greater than 0.05). In contrast, the number of ISC produced after stimulation 
      with fibroblast cytoplasm was increased significantly in the patients group (p 
      less than 0.05). These data support the hypothesis that autologous substances 
      play an important role in the cellular immune responses of patients with severe 
      chronic inflammatory periodontal disease (CIPD).
FAU - Kopp, W
AU  - Kopp W
FAU - Marggraf, E
AU  - Marggraf E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Periodontol
JT  - Journal of clinical periodontology
JID - 0425123
RN  - 0 (Immunoglobulins)
RN  - 0 (Pokeweed Mitogens)
SB  - IM
MH  - Adult
MH  - Dental Plaque/physiopathology
MH  - Female
MH  - Fibroblasts/physiology
MH  - *Hemolytic Plaque Technique
MH  - Humans
MH  - Immunity, Cellular
MH  - Immunoglobulins/analysis
MH  - *Lymphocyte Activation
MH  - Lymphocytes/immunology
MH  - Male
MH  - Middle Aged
MH  - Periodontitis/*blood/immunology
MH  - Pokeweed Mitogens/pharmacology
EDAT- 1986/10/01 00:00
MHDA- 1986/10/01 00:01
CRDT- 1986/10/01 00:00
PHST- 1986/10/01 00:00 [pubmed]
PHST- 1986/10/01 00:01 [medline]
PHST- 1986/10/01 00:00 [entrez]
AID - 10.1111/j.1600-051x.1986.tb02234.x [doi]
PST - ppublish
SO  - J Clin Periodontol. 1986 Oct;13(9):805-9. doi: 
      10.1111/j.1600-051x.1986.tb02234.x.