PMID- 35370930
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220519
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 12
DP  - 2021
TI  - Full Functional Sex Reversal Achieved Through Silencing of MroDmrt11E Gene in 
      Macrobrachium rosenbergii: Production of All-Male Monosex Freshwater Prawn.
PG  - 772498
LID - 10.3389/fendo.2021.772498 [doi]
LID - 772498
AB  - The freshwater prawn Macrobrachium rosenbergii is one kind of important economic 
      aquaculture species and displays remarkable sexual dimorphism. The molecular 
      mechanism of sexual differentiation in M. rosenbergii has been primarily 
      unraveled through the research efforts of the androgenic gland and its related 
      genes. However, the understanding of conserved genes involved in the molecular 
      mechanism underpinning sex determination and sexual differentiation of M. 
      rosenbergii is still fragmentary. MroDmrt11E is a member of the doublesex and 
      mab-3-related transcription factor (Dmrt) gene family and is prominently 
      expressed in the testis. In the present study, in vivo knockdown of MroDmrt11E at 
      the postlarva stage in male prawn induced a complete and functional sex reversal 
      and achieved the production of an all-male monosex population. Furthermore, a 
      great deal of new information of upregulated and downregulated transcriptions 
      involved in sexual differentiation of MroDmrt11E knockdown was enriched by 
      comparative transcriptomic analysis. The effects of RNAi-mediated gene knockdown 
      of MroDmrt11E on the differentially expressed and sex-related candidate genes, 
      such as transformer, fruitless, feminization, insulin-like androgenic gland gene, 
      Dmrt gene family, were primarily focused on, and their possible molecular 
      regulatory relationships in sexual differentiation were analyzed. Meanwhile, the 
      response of primary Kyoto Encyclopedia of Genes and Genomes (KEGG) biological 
      pathways was investigated to expound the potential roles of MroDmrt11E in male 
      sexual differentiation, which provided a deeper understanding of the molecular 
      regulatory network underlying sexual differentiation of M. rosenbergii. The 
      finding provided a novel sexual manipulation technique through silencing of Dmrt 
      gene family for achieving a complete and functional sex reversal and offered a 
      new insight regarding the mechanism of the Dmrt gene family in the sexual 
      differentiation of crustaceans.
CI  - Copyright (c) 2022 Xu, Chen, Wang, Luo, Li, Shen, Yang and Ma.
FAU - Xu, Hai-Jing
AU  - Xu HJ
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
FAU - Chen, Yi-Lai
AU  - Chen YL
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
FAU - Wang, Yong-Mei
AU  - Wang YM
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
FAU - Luo, Jing-Yu
AU  - Luo JY
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
FAU - Li, Jian-Wen
AU  - Li JW
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
FAU - Shen, Shuai-Qi
AU  - Shen SQ
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
AD  - College of Life Sciences, Zhejiang University, Hangzhou, China.
FAU - Yang, Jin-Shu
AU  - Yang JS
AD  - College of Life Sciences, Zhejiang University, Hangzhou, China.
FAU - Ma, Wen-Ming
AU  - Ma WM
AD  - College of Biological and Environmental Sciences, Zhejiang Wanli University, 
      Ningbo, China.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
SB  - IM
MH  - Animals
MH  - *Decapoda/physiology
MH  - Fresh Water
MH  - Male
MH  - *Palaemonidae/genetics
MH  - Sex Differentiation/genetics
MH  - Testis
PMC - PMC8970045
OTO - NOTNLM
OT  - Macrobrachium rosenbergii
OT  - MroDmrt11E gene knockdown
OT  - all-male monosex population
OT  - sex reversal
OT  - sexual differentiation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/05 06:00
MHDA- 2022/04/06 06:00
PMCR- 2021/01/01
CRDT- 2022/04/04 05:28
PHST- 2021/09/08 00:00 [received]
PHST- 2021/12/15 00:00 [accepted]
PHST- 2022/04/04 05:28 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2021.772498 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Mar 17;12:772498. doi: 
      10.3389/fendo.2021.772498. eCollection 2021.