PMID- 35371047 OWN - NLM STAT- MEDLINE DCOM- 20220408 LR - 20220520 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 13 DP - 2022 TI - Pre-Transplant Expression of CCR-2 in Kidney Transplant Recipients Is Associated With the Development of Delayed Graft Function. PG - 804762 LID - 10.3389/fimmu.2022.804762 [doi] LID - 804762 AB - BACKGROUND: Delayed graft function (DGF) leads to a reduced graft survival. Donors' features have been always considered as key pathogenic factors in this setting. The aim of our study was to evaluate the recipients' characteristics in the development of DGF. METHODS: We enrolled 932 kidney graft recipients from 466 donors; 226 recipients experienced DGF. In 290 donors, both recipients presented with early graft function (EGF, group A), in 50 both recipients experienced DGF (group B), and in 126 one recipient presented with DGF and the other with EGF (group C). In group C, we selected 7 couples of DGF/EGF recipients and we evaluated the transcriptomic profile by microarray on circulating mononuclear cells harvested before transplantation. Results were validated by qPCR in an independent group of 25 EGF/DGF couples. FINDINGS: In the whole study group, DGF was associated with clinical characteristics related to both donors and recipient. In group C, DGF was significantly associated with body mass index, hemodialysis, and number of mismatches. In the same group, we identified 411 genes differently expressed before transplantation between recipients discordant for the transplant outcome. Those genes were involved in immune dysfunction and inflammation. In particular, we observed a significant increase in DGF patients in the expression of C-C chemokine receptor type 2 (CCR2), the monocyte chemoattractant protein-1 (MCP-1) receptor. CCR-2 upregulation was confirmed in an independent cohort of patients. CONCLUSIONS: Our results suggest that recipients' clinical/immunological features, potentially modulated by dialysis, are associated with the development of DGF independently of donors' features. CI - Copyright (c) 2022 Pontrelli, Simone, Rascio, Pesce, Conserva, Infante, Castellano, Sallustio, Fiorentino, Zaza, Gallone, Battaglia, Ditonno, Stallone, Gesualdo and Grandaliano. FAU - Pontrelli, Paola AU - Pontrelli P AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Simone, Simona AU - Simone S AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Rascio, Federica AU - Rascio F AD - Department of Medical and Surgical Sciences, Renal Unit, University of Foggia, Foggia, Italy. FAU - Pesce, Francesco AU - Pesce F AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Conserva, Francesca AU - Conserva F AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Infante, Barbara AU - Infante B AD - Department of Medical and Surgical Sciences, Renal Unit, University of Foggia, Foggia, Italy. FAU - Castellano, Giuseppe AU - Castellano G AD - Department of Clinical Sciences and Community Health, Nephrology Unit, University of Milano and Fondazione Ca Grande Ospedale Maggiore Policlinico, Milano, Italy. FAU - Sallustio, Fabio AU - Sallustio F AD - Department of Internal Medicine, University of Bari Aldo Moro, Bari, Italy. FAU - Fiorentino, Marco AU - Fiorentino M AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Zaza, Gianluigi AU - Zaza G AD - Department of Medical and Surgical Sciences, Renal Unit, University of Foggia, Foggia, Italy. FAU - Gallone, Anna AU - Gallone A AD - Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, Bari, Italy. FAU - Battaglia, Michele AU - Battaglia M AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Ditonno, Pasquale AU - Ditonno P AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Stallone, Giovanni AU - Stallone G AD - Department of Medical and Surgical Sciences, Renal Unit, University of Foggia, Foggia, Italy. FAU - Gesualdo, Loreto AU - Gesualdo L AD - Department of Emergency and Organ Transplantation, Nephrology and Urology Units, University of Bari Aldo Moro, Bari, Italy. FAU - Grandaliano, Giuseppe AU - Grandaliano G AD - Department of Translational Medicine and Surgery, Nephrology Unit, Universita Cattolica del Sacro Cuore and Fondazione Policlinico Universitario "A. Gemelli", Rome, Italy. LA - eng PT - Journal Article DEP - 20220316 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Receptors, Chemokine) RN - 62229-50-9 (Epidermal Growth Factor) SB - IM MH - *Delayed Graft Function MH - Epidermal Growth Factor MH - Humans MH - *Kidney Transplantation/adverse effects/methods MH - Receptors, Chemokine MH - Risk Factors PMC - PMC8967482 OTO - NOTNLM OT - CCR-2 OT - delayed graft function OT - gene expression OT - kidney transplantation OT - peripheral blood mononuclear cells COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/04/05 06:00 MHDA- 2022/04/09 06:00 PMCR- 2022/01/01 CRDT- 2022/04/04 05:28 PHST- 2021/10/29 00:00 [received] PHST- 2022/02/11 00:00 [accepted] PHST- 2022/04/04 05:28 [entrez] PHST- 2022/04/05 06:00 [pubmed] PHST- 2022/04/09 06:00 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2022.804762 [doi] PST - epublish SO - Front Immunol. 2022 Mar 16;13:804762. doi: 10.3389/fimmu.2022.804762. eCollection 2022.