PMID- 35371261
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220405
IS  - 1687-8760 (Print)
IS  - 1687-8779 (Electronic)
IS  - 1687-8760 (Linking)
VI  - 2022
DP  - 2022
TI  - Simultaneous Determination of Fifteen Polyphenols in Fruit Juice Using 
      Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Combining 
      Dispersive Liquid-Liquid Microextraction.
PG  - 5486290
LID - 10.1155/2022/5486290 [doi]
LID - 5486290
AB  - Polyphenols are secondary metabolites of plants and used as effective 
      antioxidants in dietary supplements, whose main sources are fruits, vegetables, 
      and grains. To clarify the content and distribution of polyphenols in different 
      fruit species samples accurately, a rapid and sensitive ultrahigh-pressure liquid 
      chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) 
      method combining dispersive liquid-liquid microextraction (DLLME) was developed 
      for quantitative determination of fifteen polyphenol compounds in fruit juice. In 
      this method, the targets were first extracted from 1 g of fruit juice sample 
      using 10 mL of 80% ethanol solution by ultrasonic-assisted extraction (UAE). 
      Then, 1.0 mL of UAE extracted solution, 60 muL of n-octanol and 2.0 mL of H(2)O 
      were performed in the following DLLME procedure. A C(18) reversed-phase column, 
      ZORBAX SB (100 x 4.6 mm, 3.5 mum), was proposed under gradient elution with 0.1% 
      formic acid aqueous solution and methanol mobile phases for the determination of 
      15 polyphenols, allowing us to obtain polyphenolic profiles in less than 
      23.0 min. Under the optimum conditions, the enrichment factors ranged from 162 to 
      194. The results showed that the 15 polyphenols had linear correlation 
      coefficients (R (2)) more than 0.99. The limits of detection (LODs) were between 
      18.3 and 103.5 ng/g, and the average recoveries were between 96.9 and 116.3% with 
      interday relative standard deviations (RSDs) ranging from 4.4 to 8.2% in all 
      cases. The method was successfully applied to the analysis of real fruit juice 
      samples and presented itself as a simple, rapid, practical, and 
      environment-friendly technique.
CI  - Copyright (c) 2022 Yuxiu Li et al.
FAU - Li, Yuxiu
AU  - Li Y
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of 
      Tobacco and Health, University of Science and Technology of China, Hefei 230052, 
      China.
FAU - He, Zengyang
AU  - He Z
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Center of 
      Technology, China Tobacco Anhui Industrial Corporation, Hefei 230088, China.
FAU - Bao, Youmei
AU  - Bao Y
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of 
      Tobacco and Health, University of Science and Technology of China, Hefei 230052, 
      China.
FAU - Zhu, Qingsheng
AU  - Zhu Q
AUID- ORCID: 0000-0003-1393-2168
AD  - School of Economics and Management, Anhui Jianzhu University, Hefei 230601, 
      China.
FAU - Ning, Yong
AU  - Ning Y
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Center of 
      Technology, China Tobacco Anhui Industrial Corporation, Hefei 230088, China.
FAU - Tian, Zhenfeng
AU  - Tian Z
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Center of 
      Technology, China Tobacco Anhui Industrial Corporation, Hefei 230088, China.
FAU - Zhu, Xiaolan
AU  - Zhu X
AUID- ORCID: 0000-0001-5579-3982
AD  - The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry, Research Center of 
      Tobacco and Health, University of Science and Technology of China, Hefei 230052, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220323
PL  - Egypt
TA  - Int J Anal Chem
JT  - International journal of analytical chemistry
JID - 101519424
PMC - PMC8967586
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2022/04/05 06:00
MHDA- 2022/04/05 06:01
PMCR- 2022/03/23
CRDT- 2022/04/04 05:29
PHST- 2022/01/20 00:00 [received]
PHST- 2022/03/01 00:00 [revised]
PHST- 2022/03/08 00:00 [accepted]
PHST- 2022/04/04 05:29 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/05 06:01 [medline]
PHST- 2022/03/23 00:00 [pmc-release]
AID - 10.1155/2022/5486290 [doi]
PST - epublish
SO  - Int J Anal Chem. 2022 Mar 23;2022:5486290. doi: 10.1155/2022/5486290. eCollection 
      2022.