PMID- 35373185
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220405
IS  - 2673-3080 (Electronic)
IS  - 2673-3080 (Linking)
VI  - 4
DP  - 2022
TI  - Models of Dendritic Cells to Assess Skin Sensitization.
PG  - 851017
LID - 10.3389/ftox.2022.851017 [doi]
LID - 851017
AB  - Allergic contact dermatitis (ACD) is a complex skin pathology occurring in 
      reaction against environmental substances found in the workplace (cement, hair 
      dyes, textile dyes), in the private environment (e.g., household products, 
      cosmetic ingredients), or following skin exposure to drugs. Many cells are 
      involved in the initiation of ACD during the sensitization phase. The four key 
      events (KE) of skin sensitization AOP are covalent binding to skin proteins 
      (KE1), keratinocyte activation (KE2), activation of DCs (KE3), and T-cell 
      activation and proliferation (KE4), leading to the adverse outcome of ACD. 
      Dendritic cells (DCs) are thus playing a key role in ACD pathophysiology. Indeed, 
      in the presence of chemical sensitizers, DCs migrate from the skin to the 
      draining lymph nodes and present peptide-chemical conjugates to T cells, leading 
      to their activation and proliferation. In vitro methods have been actively 
      developed to assess the activation of DCs by chemicals to establish a reliable in 
      vitro sensitization test. Therefore, this review will detail the most used 
      methods and protocols to develop DC models in vitro. Three different models of 
      DCs will be addressed: 1) DCs derived from Cord Blood (CD34-DCs), 2) DCs derived 
      from Monocytes (Mo-DCs), and 3) DCs derived from mice Bone-Marrow (BM-DCs). In 
      addition, a model of exposition to contact sensitizers to assess KE3 of skin 
      sensitization will be detailed for each of the models presented.
CI  - Copyright (c) 2022 Hardonniere, Szely, El Ali, Pallardy and Kerdine-Romer.
FAU - Hardonniere, Kevin
AU  - Hardonniere K
AD  - Universite Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 
      Chatenay-Malabry, France.
FAU - Szely, Natacha
AU  - Szely N
AD  - Universite Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 
      Chatenay-Malabry, France.
FAU - El Ali, Zeina
AU  - El Ali Z
AD  - Universite Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 
      Chatenay-Malabry, France.
FAU - Pallardy, Marc
AU  - Pallardy M
AD  - Universite Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 
      Chatenay-Malabry, France.
FAU - Kerdine-Romer, Saadia
AU  - Kerdine-Romer S
AD  - Universite Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, 
      Chatenay-Malabry, France.
LA  - eng
PT  - Journal Article
DEP - 20220318
PL  - Switzerland
TA  - Front Toxicol
JT  - Frontiers in toxicology
JID - 101777990
PMC - PMC8971372
OTO - NOTNLM
OT  - ACD
OT  - BM-DCs
OT  - CD34-DCs
OT  - DCs
OT  - Mo-DCs
OT  - sensitization
OT  - skin
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/05 06:00
MHDA- 2022/04/05 06:01
PMCR- 2022/03/18
CRDT- 2022/04/04 05:36
PHST- 2022/01/08 00:00 [received]
PHST- 2022/02/17 00:00 [accepted]
PHST- 2022/04/04 05:36 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/05 06:01 [medline]
PHST- 2022/03/18 00:00 [pmc-release]
AID - 851017 [pii]
AID - 10.3389/ftox.2022.851017 [doi]
PST - epublish
SO  - Front Toxicol. 2022 Mar 18;4:851017. doi: 10.3389/ftox.2022.851017. eCollection 
      2022.