PMID- 35376973 OWN - NLM STAT- MEDLINE DCOM- 20220505 LR - 20220505 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 106 IP - 8 DP - 2022 Apr TI - The effect of inulin-type fructans on the intestinal immune function of antibiotic-treated mice. PG - 3265-3278 LID - 10.1007/s00253-022-11896-0 [doi] AB - This study aimed to evaluate the effect of supplementation with inulin-type fructans (ITFs) on the intestinal immune function in the context of dysbiosis resulting from antibiotic cocktail (ABx) treatment. BALB/c mice (8-9 weeks of age) were treated with an ABx for 3 weeks and then allowed to recover spontaneously or with ITF supplementation (5%) for 4 weeks. Our results showed that ABx treatment can induce gut microbiota dysbiosis and intestinal inflammation in mice. After 4 weeks of recovery, ITF supplementation restored the composition of the intestinal microbial community. However, compared with spontaneous recovery, ITF supplementation delayed inflammation recovery in the intestine and upregulated diamine oxidase (DAO) activity and increased lipopolysaccharide (LPS) content in serum. In addition, ITF supplementation delayed the regulatory T (Treg) cell and B cell recovery in the lamina propria (LP). Furthermore, compared with spontaneous recovery, ITF supplementation inhibited the relative expression of certain proinflammatory genes, such as for inducible nitric oxide synthase (iNOS) and tumour necrosis factor alpha (Tnf-alpha), in the colon, but it reduced the secretion of the anti-inflammatory mediator transforming growth factor beta1 (TGF-beta1) in serum, reduced the secretion of secretory immunoglobulin A (SIgA) in the colon and promoted the secretion of the proinflammatory cytokine interleukin (IL)-17A. In conclusion, these data supported the hypothesis that the influence of ITFs on the host's intestinal status is not always beneficial in the context of ABx-induced biological disorder. However, the significance of these findings needs to be determined by advanced studies KEY POINTS: * ITFs did not promote the recovery of microbial community composition. * ITFs delayed the recovery of ABx-induced colonic inflammation. * ITFs reduced the secretion of TGF-beta1 and SIgA. * ITFs delayed the recovery of Treg and B cells in the LP. CI - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Zeng, Wenjing AU - Zeng W AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Zhang, Qin AU - Zhang Q AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Feng, Gang AU - Feng G AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Liu, Gongliang AU - Liu G AD - College of Light Industry and Food, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. FAU - Wu, Fenglin AU - Wu F AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Shen, Han AU - Shen H AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Shao, Hongwei AU - Shao H AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. FAU - Tao, Changli AU - Tao C AUID- ORCID: 0000-0003-0398-9646 AD - Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China. taochangli@126.com. LA - eng GR - 81703053/National Natural Science Foundation of China/ GR - 2018B020206001/Key-Area Research and Development Program of Guangdong Province/ GR - 2021KJ101/Guangdong Provincial Agricultural Science and Technology Innovation and Extension Project in 2021/ GR - 2018A030313860/Natural Science Foundation of Guangdong Province/ GR - 2017KZDXM049/Innovative and Strong School Project of Guangdong Higher Education Institutions/ GR - 2017KCXTD020/Innovative and Strong School Project of Guangdong Higher Education Institutions/ PT - Journal Article DEP - 20220404 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Anti-Bacterial Agents) RN - 0 (Fructans) RN - 0 (Immunoglobulin A, Secretory) RN - 0 (Transforming Growth Factor beta1) RN - 9005-80-5 (Inulin) SB - IM MH - Animals MH - Anti-Bacterial Agents/adverse effects MH - Dysbiosis MH - *Fructans/pharmacology MH - Immunity MH - Immunoglobulin A, Secretory MH - Inflammation MH - Intestines MH - *Inulin/pharmacology MH - Mice MH - Mice, Inbred BALB C MH - Transforming Growth Factor beta1 OTO - NOTNLM OT - Antibiotic cocktail (ABx) OT - Gut microbiota OT - Immunity OT - Inulin-type fructans EDAT- 2022/04/05 06:00 MHDA- 2022/05/06 06:00 CRDT- 2022/04/04 12:01 PHST- 2021/06/01 00:00 [received] PHST- 2022/03/19 00:00 [accepted] PHST- 2022/03/09 00:00 [revised] PHST- 2022/04/05 06:00 [pubmed] PHST- 2022/05/06 06:00 [medline] PHST- 2022/04/04 12:01 [entrez] AID - 10.1007/s00253-022-11896-0 [pii] AID - 10.1007/s00253-022-11896-0 [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2022 Apr;106(8):3265-3278. doi: 10.1007/s00253-022-11896-0. Epub 2022 Apr 4.