PMID- 35377355
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220507
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 181
DP  - 2022 Mar 16
TI  - High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine 
      Hindlimb Gangrene Model.
LID - 10.3791/63284 [doi]
AB  - Peripheral arterial disease (PAD) is a significant cause of morbidity resulting 
      from chronic exposure to atherosclerotic risk factors. Patients suffering from 
      its most severe form, chronic limb-threatening ischemia (CLTI), face substantial 
      impairments to daily living, including chronic pain, limited walking distance 
      without pain, and nonhealing wounds. Preclinical models have been developed in 
      various animals to study PAD, but mouse hindlimb ischemia remains the most widely 
      used. There can be significant variation in response to ischemic insult in these 
      models depending on the mouse strain used and the site, number, and means of 
      arterial disruption. This protocol describes a unique method combining femoral 
      artery and vein electrocoagulation with the administration of a nitric oxide 
      synthase (NOS) inhibitor to reliably induce footpad gangrene in Friend Virus B 
      (FVB) mice that resembles the tissue loss of CLTI. While traditional means of 
      assessing reperfusion such as laser Doppler perfusion imaging (LDPI) are still 
      recommended, intracardiac perfusion of the lipophilic dye 
      1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is used 
      to label the vasculature. Subsequent whole-mount confocal laser scanning 
      microscopy allows for high-resolution, three-dimensional (3D) reconstruction of 
      footpad vascular networks that complements traditional means of assessing 
      reperfusion in hindlimb ischemia models.
FAU - Ribieras, Antoine J
AU  - Ribieras AJ
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine.
FAU - Ortiz, Yulexi Y
AU  - Ortiz YY
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine; Vascular Biology Institute, University of Miami Miller School of 
      Medicine.
FAU - Shrestha, Sophie
AU  - Shrestha S
AD  - University of Miami.
FAU - Huerta, C Theodore
AU  - Huerta CT
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine.
FAU - Shao, Hongwei
AU  - Shao H
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine; Vascular Biology Institute, University of Miami Miller School of 
      Medicine.
FAU - Boulina, Maria E
AU  - Boulina ME
AD  - Analytical Imaging Core Facility, University of Miami.
FAU - Vazquez-Padron, Roberto I
AU  - Vazquez-Padron RI
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine; Vascular Biology Institute, University of Miami Miller School of 
      Medicine.
FAU - Liu, Zhao-Jun
AU  - Liu ZJ
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine; Vascular Biology Institute, University of Miami Miller School of 
      Medicine; zliu@med.miami.edu.
FAU - Velazquez, Omaida C
AU  - Velazquez OC
AD  - Dewitt Daughtry Family Department of Surgery, University of Miami Miller School 
      of Medicine; Vascular Biology Institute, University of Miami Miller School of 
      Medicine; ovelazquez@med.miami.edu.
LA  - eng
GR  - R01 HL149452/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20220316
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Femoral Artery
MH  - *Gangrene/diagnostic imaging
MH  - Hindlimb/blood supply/diagnostic imaging
MH  - Humans
MH  - *Imaging, Three-Dimensional
MH  - Lower Extremity
MH  - Mice
EDAT- 2022/04/05 06:00
MHDA- 2022/04/07 06:00
CRDT- 2022/04/04 12:15
PHST- 2022/04/04 12:15 [entrez]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
AID - 10.3791/63284 [doi]
PST - epublish
SO  - J Vis Exp. 2022 Mar 16;(181). doi: 10.3791/63284.