PMID- 35377532
OWN - NLM
STAT- MEDLINE
DCOM- 20220715
LR  - 20220722
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 110
IP  - 9
DP  - 2022 Sep
TI  - Study on the difference of osteogenesis and Notch signaling pathway expression in 
      biphasic calcium-phosphorus ceramic granule materials with different 
      microstructure.
PG  - 2028-2038
LID - 10.1002/jbm.b.35057 [doi]
AB  - Different microstructures including micropore diameter, micropore volume, and 
      micropore area of biphasic calcium phosphate (BCP, hydroxyapatite: beta-tricalcium 
      phosphate = 8:2) ceramics granules were obtained by varying their sintering 
      temperatures. Sprague-Dawley rat bone marrow-derived stem cells (BMSCs) were 
      co-cultured with BCPs in vitro study and the BMSCs showed different degrees of 
      proliferative activity under the influence of three materials. Cell proliferation 
      and vitality were assessed. Three kinds of BCPs were implanted in the dorsal 
      muscle of beagle dogs. At 1, 2, and 3 months, histological analyses were 
      conducted to estimate the rate of osteogenesis. Expression of Notch pathway genes 
      and osteogenic-related genes were detected by quantitative real-time polymerase 
      chain reaction (q-rtPCR). The proportion of osteogenesis area increased 
      to:48.75 +/- 4.20%, 29.48 +/- 1.55%, and 26.58 +/- 3.86% at 3 months after the 
      implantation (1050, 1150, 1250). Significant differences were observed in the 
      upregulation of Notch pathway genes among different BCPs. BCPs with different 
      micropore diameters have different ectopic osteogenesis effects and led to 
      up-regulation of the Notch signaling pathway genes to different extents.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Zhang, Dong
AU  - Zhang D
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Guo, Xiaoshuang
AU  - Guo X
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Zong, Xianlei
AU  - Zong X
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Du, Hong
AU  - Du H
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Zhao, Jingyi
AU  - Zhao J
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Du, Le
AU  - Du L
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Cao, Chunyan
AU  - Cao C
AD  - Animal Lab Center, Plastic Surgery Hospital, Peking Union Medical College, 
      Chinese Academy of Medical Sciences, Beijing, China.
FAU - Jin, Xiaolei
AU  - Jin X
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
FAU - Song, Guodong
AU  - Song G
AD  - 16th Department, Plastic Surgery Hospital, Peking Union Medical College, Chinese 
      Academy of Medical Sciences, Beijing, China.
LA  - eng
GR  - B2018007/Fund of Peking Union Medical College/
GR  - Young Scientists Fund/
GR  - 81400565/National Natural Science Foundation of China/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220404
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Calcium Phosphates)
RN  - 27YLU75U4W (Phosphorus)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Calcium/pharmacology
MH  - Calcium Phosphates/chemistry
MH  - Cell Differentiation
MH  - Ceramics/chemistry/pharmacology
MH  - Dogs
MH  - *Osteogenesis
MH  - Phosphorus/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
OTO - NOTNLM
OT  - biomaterial
OT  - bone
OT  - ceramic
OT  - material availability
OT  - microstructure
OT  - sintering
EDAT- 2022/04/05 06:00
MHDA- 2022/07/16 06:00
CRDT- 2022/04/04 12:16
PHST- 2022/02/27 00:00 [revised]
PHST- 2021/10/28 00:00 [received]
PHST- 2022/03/03 00:00 [accepted]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/07/16 06:00 [medline]
PHST- 2022/04/04 12:16 [entrez]
AID - 10.1002/jbm.b.35057 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2022 Sep;110(9):2028-2038. doi: 
      10.1002/jbm.b.35057. Epub 2022 Apr 4.