PMID- 35378356
OWN - NLM
STAT- MEDLINE
DCOM- 20220502
LR  - 20220502
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 244
DP  - 2022 Jul 1
TI  - Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: 
      Determination of IgE and APOE in aqueous humor by inductively coupled plasma-mass 
      spectrometry.
PG  - 123424
LID - S0039-9140(22)00220-X [pii]
LID - 10.1016/j.talanta.2022.123424 [doi]
AB  - Iridium nanoclusters (IrNCs) stabilized with citrate were synthesized and then 
      the ligand was exchanged by lipoic acid (LA). The IrNCs@LA were bioconjugated 
      through carbodiimide chemistry with specific antibodies to prepare IrNCs-labelled 
      immunoprobes. The IrNCs-immunoprobes were employed in competitive immunoassays 
      for immunoglobulin E (IgE) and apolipoprotein E (APOE) determination by detecting 
      the iridium through inductively coupled plasma - mass spectrometry (ICP-MS). The 
      IrNCs@LA have a 1.89 nm diameter at average and each NC contains 250 Ir atoms. 
      Labelling of specific antibodies with IrNCs was optimized in terms of recognition 
      capabilities and signal amplification by ICP-MS. Amplification and detection 
      limits can be tuned by selecting the IrNCs:Ab molar ratio. An immunoprobe 
      prepared by mixing a 10:1 IrNC:Ab molar ratio was selected for the determination 
      of IgE and APOE in aqueous humor, achieving a signal amplification of 1760 
      iridium atoms per molecule of the sought protein and limits of detection in the 
      tens of pg mL(-1) of protein. The IrNCs-immunoprobes were evaluated for IgE 
      determination in serum samples as well as for IgE and APOE in aqueous humor (from 
      controls subjects and patients affected by primary open angle glaucoma) by 
      ICP-MS, being required just sample dilution as pre-treatment. Results were 
      corroborated with commercial ELISA kits.
CI  - Copyright (c) 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Menero-Valdes, Paula
AU  - Menero-Valdes P
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain.
FAU - Lores-Padin, Ana
AU  - Lores-Padin A
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain.
FAU - Fernandez, Beatriz
AU  - Fernandez B
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain; Instituto Universitario Fernandez-Vega, 
      Fundacion de Investigacion Oftalmologica, Universidad de Oviedo, Oviedo, Spain. 
      Electronic address: fernandezbeatriz@uniovi.es.
FAU - Gonzalez-Iglesias, Hector
AU  - Gonzalez-Iglesias H
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain; Instituto Universitario Fernandez-Vega, 
      Fundacion de Investigacion Oftalmologica, Universidad de Oviedo, Oviedo, Spain; 
      Department of Technology and Biotechnology of Dairy Products, Instituto de 
      Productos Lacteos de Asturias, Consejo Superior de Investigaciones Cientificas 
      (IPLA-CSIC), Villaviciosa, Spain.
FAU - Pereiro, Rosario
AU  - Pereiro R
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain; Instituto Universitario Fernandez-Vega, 
      Fundacion de Investigacion Oftalmologica, Universidad de Oviedo, Oviedo, Spain. 
      Electronic address: mrpereiro@uniovi.es.
LA  - eng
PT  - Journal Article
DEP - 20220329
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Apolipoproteins E)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 44448S9773 (Iridium)
SB  - IM
MH  - Apolipoproteins E
MH  - Aqueous Humor
MH  - *Glaucoma, Open-Angle
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin E
MH  - *Iridium
MH  - Mass Spectrometry/methods
OTO - NOTNLM
OT  - APOE
OT  - Elemental mass spectrometry
OT  - Immunoglobulin E
OT  - Iridium nanoclusters
OT  - Metal-labelled immunoprobe
OT  - Primary open angle glaucoma
EDAT- 2022/04/05 06:00
MHDA- 2022/05/03 06:00
CRDT- 2022/04/04 20:11
PHST- 2022/01/14 00:00 [received]
PHST- 2022/03/16 00:00 [revised]
PHST- 2022/03/28 00:00 [accepted]
PHST- 2022/04/05 06:00 [pubmed]
PHST- 2022/05/03 06:00 [medline]
PHST- 2022/04/04 20:11 [entrez]
AID - S0039-9140(22)00220-X [pii]
AID - 10.1016/j.talanta.2022.123424 [doi]
PST - ppublish
SO  - Talanta. 2022 Jul 1;244:123424. doi: 10.1016/j.talanta.2022.123424. Epub 2022 Mar 
      29.