PMID- 35380303
OWN - NLM
STAT- MEDLINE
DCOM- 20220418
LR  - 20220418
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 24
IP  - 2
DP  - 2022 Apr
TI  - Production of Germ Cell-Less Rainbow Trout by dead end Gene Knockout and their 
      Use as Recipients for Germ Cell Transplantation.
PG  - 417-429
LID - 10.1007/s10126-022-10128-w [doi]
AB  - In germ cell transplantation experiments, the use of sterile recipients that do 
      not produce their own gametes is an important prerequisite. Triploidization and 
      dnd gene knockdown (KD) methods have been widely used to produce sterile fish. 
      However, triploidization does not produce complete sterility in some fish 
      species, and gene KD is labor and time intensive since it requires microinjection 
      into individual fertilized eggs. To overcome these problems, in this study, we 
      generated homozygous mutants of the dead end (dnd) gene in rainbow trout 
      (Oncorhynchus mykiss) using the clustered regulatory interspaced short 
      palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system, analyzed 
      their reproductive capacity, and evaluated their suitability as recipients for 
      germ cell transplantation. By crossing F1 heterozygous mutants produced from 
      founders subjected to genome editing, an F2 generation consisting of 
      approximately 1/4 homozygous knockout mutants (dnd KO) was obtained. The dnd KO 
      hatchlings retained the same number of primordial germ cells (PGCs) as the 
      wild-type (WT) individuals, after which the number gradually decreased. At 1 year 
      of age, germ cells were completely absent in all analyzed individuals. To 
      evaluate the dnd KO individuals as recipients for germ cell transplantation, germ 
      cells prepared from donor individuals were transplanted into the abdominal cavity 
      of dnd KO hatchlings. These cells migrated to the recipient gonads, where they 
      initiated gametogenesis. The mature recipient individuals produced only 
      donor-derived sperm and eggs in equivalent numbers to WT rainbow trout. These 
      results indicate that dnd KO rainbow trout are suitable recipient candidates 
      possessing a high capacity to nurse donor-derived germ cells.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Fujihara, Ryo
AU  - Fujihara R
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
FAU - Katayama, Naoto
AU  - Katayama N
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
FAU - Sadaie, Sakiko
AU  - Sadaie S
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
FAU - Miwa, Misako
AU  - Miwa M
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
FAU - Sanchez Matias, Gabriela Angelica
AU  - Sanchez Matias GA
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
FAU - Ichida, Kensuke
AU  - Ichida K
AD  - Institute for Reproductive Biotechnology for Aquatic Species (IRBAS), Tokyo 
      University of Marine Science and Technology, 4-5-7 Konan Minato-ku, Tokyo, 
      108-8477, Japan.
FAU - Fujii, Wataru
AU  - Fujii W
AD  - Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate 
      School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
FAU - Naito, Kunihiko
AU  - Naito K
AD  - Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate 
      School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
FAU - Hayashi, Makoto
AU  - Hayashi M
AD  - Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance 
      (TARA), University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan. 
      makotoh@tara.tsukuba.ac.jp.
FAU - Yoshizaki, Goro
AU  - Yoshizaki G
AUID- ORCID: 0000-0003-1207-1095
AD  - Department of Marine Biosciences, Tokyo University of Marine Science and 
      Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan. goro@kaiyodai.ac.jp.
AD  - Institute for Reproductive Biotechnology for Aquatic Species (IRBAS), Tokyo 
      University of Marine Science and Technology, 4-5-7 Konan Minato-ku, Tokyo, 
      108-8477, Japan. goro@kaiyodai.ac.jp.
LA  - eng
GR  - 18H05545/Ministry of Education, Culture, Sports, Science and Technology/
GR  - 20H00430/Ministry of Education, Culture, Sports, Science and Technology/
GR  - JPMJMI21C1/Japan Science and Technology Agency/
PT  - Journal Article
DEP - 20220405
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
SB  - IM
MH  - Animals
MH  - Cell Transplantation/methods
MH  - Gene Knockout Techniques
MH  - Germ Cells/transplantation
MH  - Gonads
MH  - *Infertility
MH  - *Oncorhynchus mykiss/genetics
OTO - NOTNLM
OT  - Genome editing
OT  - Spermatogonial transplantation
OT  - Sterile fish
OT  - Surrogate broodstock
EDAT- 2022/04/06 06:00
MHDA- 2022/04/19 06:00
CRDT- 2022/04/05 12:04
PHST- 2021/10/11 00:00 [received]
PHST- 2022/03/25 00:00 [accepted]
PHST- 2022/04/06 06:00 [pubmed]
PHST- 2022/04/19 06:00 [medline]
PHST- 2022/04/05 12:04 [entrez]
AID - 10.1007/s10126-022-10128-w [pii]
AID - 10.1007/s10126-022-10128-w [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2022 Apr;24(2):417-429. doi: 10.1007/s10126-022-10128-w. 
      Epub 2022 Apr 5.