PMID- 35381784
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1998-4138 (Electronic)
IS  - 1998-4138 (Linking)
VI  - 18
IP  - 1
DP  - 2022 Jan-Mar
TI  - Monte Carlo simulation for assessing absorbed dose effects of low-dose 
      beta-radiation ((90)Sr/(90)Y) on cytotoxicity and apoptotic death in K562 cells.
PG  - 200-208
LID - 10.4103/jcrt.JCRT_909_20 [doi]
AB  - OBJECTIVE: Most studies suggest that dose-specific radiation regimens are 
      essential for optimal induction of cancer cell response. This study focused on 
      determining beta-radiation-absorbed dose (rad) effects on the cell viability, 
      cytotoxicity, hypersensitivity, and cell death of K562 cells using experimental 
      methods and Monte Carlo simulation (MCS). MATERIALS AND METHODS: The K562 cells 
      were cultured and irradiated with beta-particles emitted from a strontium source in 
      vitro, with the estimated daily activity of 1.238 muCi. The treated cells were 
      radiated at least three times every day for 3 consecutive days. The cell 
      viability and apoptosis were investigated in treated cells by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, DNA 
      electrophoresis, Hoechst dye, and inverted microscope. The average absorbed doses 
      were obtained by MCS (MCNPX code). To verify simulation and experimental results, 
      we used a Geiger-Muller counter and estimated a scaling factor. RESULTS: The 
      cytotoxic effects and cell death were induced in the treated groups via rad in a 
      time-dependent manner. The highest apoptotic and cytotoxic effects were observed 
      in cells after irradiation with beta-particles for 120 min per day in 3 consecutive 
      days. rads were determined using MCNPX code and cell survival rates were 
      significantly reduced during irradiation periods. No significant 
      hyper-radiosensitivity was found based on experimental and theoretical results. 
      CONCLUSION: Despite the difficult calculation of the rad in the target cells and 
      the scant information in this field, fortunately we have achieved significant 
      theoretical data consistent with the experimental results. Our findings also 
      introduced MCS as a better choice for evaluating of rad effects under different 
      cellular conditions with high accuracy.
FAU - Alizadeh, Zeynab
AU  - Alizadeh Z
AD  - Department of Physics, Urmia University, Urmia, Iran.
FAU - Khodabakhsh, Rasoul
AU  - Khodabakhsh R
AD  - Department of Physics, Urmia University, Urmia, Iran.
FAU - Mohammadzadeh, Mehdi
AU  - Mohammadzadeh M
AD  - Department of Biology, Urmia University, Urmia, Iran.
FAU - Pazhang, Yaghub
AU  - Pazhang Y
AD  - Department of Biology, Urmia University, Urmia, Iran.
FAU - Mohammadi, Seyed Mostafa
AU  - Mohammadi SM
AD  - Department of Physics, Urmia University, Urmia, Iran.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Cancer Res Ther
JT  - Journal of cancer research and therapeutics
JID - 101249598
RN  - 0 (Strontium Radioisotopes)
RN  - 5TZZ77Z4ER (Strontium-90)
SB  - IM
MH  - Computer Simulation
MH  - Humans
MH  - K562 Cells
MH  - Monte Carlo Method
MH  - Radiation Dosage
MH  - *Radiometry/methods
MH  - *Strontium Radioisotopes
OTO - NOTNLM
OT  - Apoptotic death
OT  - Monte Carlo simulation
OT  - chronic myeloid leukemia
OT  - cytotoxicity
OT  - low-dose beta-radiation
OT  - radiation-absorbed dose
COIS- None
EDAT- 2022/04/07 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/04/06 03:31
PHST- 2022/04/06 03:31 [entrez]
PHST- 2022/04/07 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
AID - JCanResTher_2022_18_1_200_341170 [pii]
AID - 10.4103/jcrt.JCRT_909_20 [doi]
PST - ppublish
SO  - J Cancer Res Ther. 2022 Jan-Mar;18(1):200-208. doi: 10.4103/jcrt.JCRT_909_20.