PMID- 35382644
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20231213
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 41
DP  - 2022 Jan-Dec
TI  - A comparative assessment of e-cigarette aerosol extracts and tobacco cigarette 
      smoke extracts on in vitro endothelial cell inflammation response.
PG  - 9603271221088996
LID - 10.1177/09603271221088996 [doi]
AB  - With the development of the times, electronic cigarettes (e-cigarettes) are being 
      received by more and more people. We compared the different effects of 
      e-cigarettes and tobacco cigarettes on human umbilical vein endothelial cells 
      (HUVECs) treated with the typical e-cigarette aerosol extracts (ECA) and 
      cigarette smoking extracts (CS) sourced from commercial retail stores. HUVECs 
      were treated with different kinds of ECA or CS with different nicotinic 
      concentrations (0.03125, 0.125, 0.5, 2, 8, or 32 mug/mL). Cell viability was 
      examined by the MTT assay. The cell apoptosis was investigated by acridine orange 
      (AO) and Hoechst 33258 staining. The RT-PCR and western blot assays were used to 
      analyze the adhesion molecules and inflammation cytokines released by HUVECs. 
      Furthermore, the intracellular reactive oxygen species (ROS) was observed by 
      fluorescence microscopy. Our data showed that the CS (nicotine concentration at 
      0.125 mug/mL could decrease the viability of HUVECs by 71%, but not the four kinds 
      of ECA. The apoptotic ratio was about 32.5% in the CS group. No matter the levels 
      of adhesion molecules, inflammation cytokines or ROS, they were higher in CS 
      groups than in ECA groups. Overall, the four kinds of e-cigarettes induced 
      significantly less cytotoxicity than the commercially available tobacco 
      cigarettes in HUVECs. The CS showed the most severe impact on HUVECs. ECA might 
      provide a harm reduction measure, especially in cardiovascular risk, after people 
      switch from tobacco cigarettes to e-cigarettes.
FAU - Su, Le
AU  - Su L
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Zhao, Minghan
AU  - Zhao M
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Ma, Feifan
AU  - Ma F
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - An, Zaiyong
AU  - An Z
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Yue, Qiulin
AU  - Yue Q
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Zhao, Chen
AU  - Zhao C
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Sun, Xin
AU  - Sun X
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Zhang, Song
AU  - Zhang S
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
FAU - Xu, Jing
AU  - Xu J
AD  - Shenzhen RELX Tech. Co., Ltd, Shenzhen, China.
FAU - Jiang, Xingtao
AU  - Jiang X
AD  - Shenzhen RELX Tech. Co., Ltd, Shenzhen, China.
FAU - Li, Kunlun
AU  - Li K
AD  - Jinan Hangchen Biotechnology Co., Ltd, Jinan, China.
FAU - Zhao, Lin
AU  - Zhao L
AUID- ORCID: 0000-0002-4016-3176
AD  - State Key Laboratory of Biobased Material and Green Papermaking, School of 
      Bioengineering, 12689Qilu University of Technology, Shandong Academy of Sciences, 
      Jinan, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Aerosols)
SB  - IM
MH  - Aerosols
MH  - *Cigarette Smoking
MH  - *Electronic Nicotine Delivery Systems
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Inflammation
MH  - Nicotiana/toxicity
OTO - NOTNLM
OT  - Electronic cigarette
OT  - apoptosis
OT  - human umbilical vein endothelial cells
OT  - inflammation
OT  - tobacco cigarette
EDAT- 2022/04/07 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/04/06 05:20
PHST- 2022/04/06 05:20 [entrez]
PHST- 2022/04/07 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
AID - 10.1177/09603271221088996 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2022 Jan-Dec;41:9603271221088996. doi: 
      10.1177/09603271221088996.