PMID- 35382824
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220531
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 20
IP  - 1
DP  - 2022 Apr 5
TI  - Circ_0036412 affects the proliferation and cell cycle of hepatocellular carcinoma 
      via hedgehog signaling pathway.
PG  - 154
LID - 10.1186/s12967-022-03305-x [doi]
LID - 154
AB  - BACKGROUND: Hepatocellular carcinoma (HCC), as the most common type of liver 
      cancer, is characterized by high recurrence and metastasis. Circular RNA 
      (circRNA) circ_0036412 was selected for studying the underlying mechanisms of 
      HCC. METHODS: Quantitative real time-polymerase chain reaction (qRT-PCR) and 
      western blot analyzed gene and protein expression. Functional experiments 
      evaluated HCC cell proliferation, apoptosis and cell cycle in vitro. In vivo 
      experiments detected HCC carcinogenesis in vivo. Fluorescence in situ 
      hybridization (FISH) assays evaluated the subcellular distribution. Luciferase 
      reporter, Chromatin immunoprecipitation (ChIP), DNA pulldown, RNA-binding protein 
      immunoprecipitation (RIP), and RNA pulldown assays detected the underlying 
      mechanisms. RESULTS: Circ_0036412 is overexpressed in HCC cells and features 
      circular structure. PRDM1 activates circ_0036412 transcription to regulate the 
      proliferation and cell cycle of HCC cells in vitro. Circ_0036412 modulates 
      Hedgehog pathway. GLI2 propels HCC growth in vivo. Circ_0036412 up-regulates GLI2 
      expression by competitively binding to miR-579-3p, thus promoting the 
      proliferation and inhibiting cell cycle arrest of HCC cells. Circ_0036412 
      stabilizes GLI2 expression by recruiting ELAVL1. Circ_0036412 propels the 
      proliferation and inhibits cell cycle arrest of HCC cells in vitro through 
      Hedgehog pathway. CONCLUSIONS: Circ_0036412 affects the proliferation and cell 
      cycle of HCC via Hedgehog signaling pathway. It offers an insight into the 
      targeted therapies of HCC.
CI  - (c) 2022. The Author(s).
FAU - Wang, Liyan
AU  - Wang L
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China.
FAU - Li, Bin
AU  - Li B
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China. 
      Leebien@163.com.
FAU - Yi, Xiaoyuan
AU  - Yi X
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China.
FAU - Xiao, Xuhua
AU  - Xiao X
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China.
FAU - Zheng, Qinghua
AU  - Zheng Q
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China.
FAU - Ma, Lei
AU  - Ma L
AD  - Department of Gastroenterology, Affiliated Hospital of Guilin Medical College, 
      No. 15 Lequn Road, Xiufeng District, Guilin, 541001, Guangxi, China.
LA  - eng
PT  - Journal Article
DEP - 20220405
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Hedgehog Proteins)
RN  - 0 (MIRN579 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - *Carcinoma, Hepatocellular/pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Hedgehog Proteins/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Liver Neoplasms/pathology
MH  - *MicroRNAs/genetics
MH  - *RNA, Circular/genetics
PMC - PMC8981839
OTO - NOTNLM
OT  - GLI2
OT  - HCC
OT  - Hedgehog signaling pathway
OT  - circ_0036412
COIS- The authors declare that they have no competing interests.
EDAT- 2022/04/07 06:00
MHDA- 2022/04/08 06:00
PMCR- 2022/04/05
CRDT- 2022/04/06 05:21
PHST- 2021/11/28 00:00 [received]
PHST- 2022/02/12 00:00 [accepted]
PHST- 2022/04/06 05:21 [entrez]
PHST- 2022/04/07 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/04/05 00:00 [pmc-release]
AID - 10.1186/s12967-022-03305-x [pii]
AID - 3305 [pii]
AID - 10.1186/s12967-022-03305-x [doi]
PST - epublish
SO  - J Transl Med. 2022 Apr 5;20(1):154. doi: 10.1186/s12967-022-03305-x.