PMID- 35385114
OWN - NLM
STAT- MEDLINE
DCOM- 20220629
LR  - 20230407
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 189
IP  - 3
DP  - 2022 Jun 27
TI  - PDAT regulates PE as transient carbon sink alternative to triacylglycerol in 
      Nannochloropsis.
PG  - 1345-1362
LID - 10.1093/plphys/kiac160 [doi]
AB  - Triacylglycerols (TAGs) are the main storage lipids in photosynthetic organisms 
      under stress. In the oleaginous alga Nannochloropsis oceanica, while multiple 
      acyl CoA:diacylglycerol (DAG) acyltransferases (NoDGATs) are involved in TAG 
      production, the role of the unique phospholipid:DAG acyltransferase (NoPDAT) 
      remains unknown. Here, we performed a functional complementation assay in 
      TAG-deficient yeast (Saccharomyces cerevisiae) and an in vitro assay to probe the 
      acyltransferase activity of NoPDAT. Subcellular localization, overexpression, and 
      knockdown (KD) experiments were also conducted to elucidate the role of NoPDAT in 
      N. oceanica. NoPDAT, residing at the outermost plastid membrane, does not 
      phylogenetically fall into the clades of algae or plants and uses 
      phosphatidylethanolamine (PE) and phosphatidylglycerol with 16:0, 16:1, and 18:1 
      at position sn-2 as acyl-donors in vivo. NoPDAT KD, not triggering any 
      compensatory mechanism via DGATs, led to an  approximately 30% decrease of TAG content, 
      accompanied by a vast accumulation of PEs rich in 16:0, 16:1, and 18:1 fatty 
      acids (referred to as "LU-PE") that was positively associated with CO2 
      availability. We conclude that the NoPDAT pathway is parallel to and independent 
      of the NoDGAT pathway for oil production. LU-PE can serve as an alternative 
      carbon sink for photosynthetically assimilated carbon in N. oceanica when 
      PDAT-mediated TAG biosynthesis is compromised or under stress in the presence of 
      high CO2 levels.
CI  - (c) American Society of Plant Biologists 2022. All rights reserved. For 
      permissions, please email: journals.permissions@oup.com.
FAU - Yang, Juan
AU  - Yang J
AUID- ORCID: 0000-0003-1736-1964
AD  - Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of 
      Sciences, Wuhan 430072, China.
AD  - College of Advanced Agricultural Sciences, University of Chinese Academy of 
      Sciences, Beijing 100049, China.
FAU - Liu, Jin
AU  - Liu J
AUID- ORCID: 0000-0002-4050-7207
AD  - Laboratory for Algae Biotechnology and Innovation, College of Engineering, Peking 
      University, Beijing 100871, China.
FAU - Pan, Yufang
AU  - Pan Y
AUID- ORCID: 0000-0003-0150-1264
AD  - Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of 
      Sciences, Wuhan 430072, China.
FAU - Marechal, Eric
AU  - Marechal E
AD  - Laboratoire de Physiologie Cellulaire Vegetale, Universite Grenoble Alpes, CEA, 
      CNRS, INRA, IRIG-LPCV, 38054 Grenoble Cedex 9, France.
FAU - Amato, Alberto
AU  - Amato A
AUID- ORCID: 0000-0002-9126-5535
AD  - Laboratoire de Physiologie Cellulaire Vegetale, Universite Grenoble Alpes, CEA, 
      CNRS, INRA, IRIG-LPCV, 38054 Grenoble Cedex 9, France.
FAU - Liu, Meijing
AU  - Liu M
AUID- ORCID: 0000-0003-4574-1358
AD  - Laboratory for Algae Biotechnology and Innovation, College of Engineering, Peking 
      University, Beijing 100871, China.
FAU - Gong, Yangmin
AU  - Gong Y
AUID- ORCID: 0000-0002-4096-3640
AD  - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of 
      Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural 
      Sciences, Wuhan 430062, China.
FAU - Li, Yantao
AU  - Li Y
AUID- ORCID: 0000-0001-7545-1883
AD  - Institute of Marine and Environmental Technology, University of Maryland Center 
      for Environmental Science and University of Maryland Baltimore County, Baltimore, 
      Maryland 21202, USA.
FAU - Hu, Hanhua
AU  - Hu H
AUID- ORCID: 0000-0002-8485-7591
AD  - Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of 
      Sciences, Wuhan 430072, China.
AD  - College of Advanced Agricultural Sciences, University of Chinese Academy of 
      Sciences, Beijing 100049, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Triglycerides)
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (phospholipid diacylglycerol acyltransferase)
RN  - EC 2.3.1.20 (Diacylglycerol O-Acyltransferase)
SB  - IM
MH  - *Acyltransferases/genetics/metabolism
MH  - Carbon Dioxide/metabolism
MH  - Carbon Sequestration/genetics/physiology
MH  - Diacylglycerol O-Acyltransferase/metabolism
MH  - *Microalgae/genetics/metabolism
MH  - *Phosphatidylethanolamines/genetics/metabolism
MH  - Plants/metabolism
MH  - Saccharomyces cerevisiae/genetics/metabolism
MH  - Triglycerides/genetics/metabolism
PMC - PMC9237688
EDAT- 2022/04/07 06:00
MHDA- 2022/06/30 06:00
PMCR- 2023/04/06
CRDT- 2022/04/06 12:16
PHST- 2022/02/23 00:00 [received]
PHST- 2022/03/14 00:00 [accepted]
PHST- 2022/04/07 06:00 [pubmed]
PHST- 2022/06/30 06:00 [medline]
PHST- 2022/04/06 12:16 [entrez]
PHST- 2023/04/06 00:00 [pmc-release]
AID - 6564233 [pii]
AID - kiac160 [pii]
AID - 10.1093/plphys/kiac160 [doi]
PST - ppublish
SO  - Plant Physiol. 2022 Jun 27;189(3):1345-1362. doi: 10.1093/plphys/kiac160.