PMID- 35385208
OWN - NLM
STAT- MEDLINE
DCOM- 20220609
LR  - 20220621
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 37
IP  - 7
DP  - 2022 Jul
TI  - Leydig cell function in adult male rats is disrupted by perfluorotetradecanoic 
      acid through increasing oxidative stress and apoptosis.
PG  - 1790-1802
LID - 10.1002/tox.23526 [doi]
AB  - Perfluorotetradecanoic acid (PFTeDA) is a long-chain perfluoroalkyl compound with 
      increased applications. Its effect on Leydig cell function and its underlying 
      mechanism remain unclear. Male Sprague-Dawley rats (60 days old) were gavaged 
      with PFTeDA at doses of 0, 1, 5, and 10 mg/kg/day from 60 to 87 days after birth. 
      PFTeDA significantly reduced serum testosterone levels at 1 mg/kg and higher 
      doses, while markedly increasing serum luteinizing hormone level at 10 mg/kg and 
      follicle-stimulating hormone at >/=1 mg/kg. PFTeDA significantly reduced the sperm 
      number at the cauda of epididymis at >/=1 mg/kg. PFTeDA also reduced the number of 
      CYP11A1-positive Leydig cells due to increased apoptosis shown by the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. 
      PFTeDA significantly repressed the expression of Cyp17a1 and Star and their 
      proteins at 1-10 mg/kg, while it increased the expression of Srd5a1 and its 
      protein (an immature Leydig cell biomarker) at 10 mg/kg. PFTeDA markedly 
      increased testicular malondialdehyde level, while inhibiting antioxidants (SOD1, 
      SOD2, and CAT), triggering oxidative stress, thereby further inducing BAX and 
      CASP3 while inhibiting BCL2, which led to cell apoptosis. PFTeDA also reduced DHH 
      level secreted by Sertoli cells, which indirectly affected Leydig cell function. 
      PFTeDA inhibited testosterone secretion in primary Leydig cells in vitro by 
      increasing reactive oxygen species and inducing apoptosis at 50 muM. In 
      conclusion, PFTeDA inhibits the function of Leydig cells by inducing oxidative 
      stress and subsequently stimulating cell apoptosis.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Tang, Yunbing
AU  - Tang Y
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Ying, Yingfen
AU  - Ying Y
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Zou, Cheng
AU  - Zou C
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Yan, Haoni
AU  - Yan H
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Wang, Yiyan
AU  - Wang Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Li, Huitao
AU  - Li H
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Li, Xiaoheng
AU  - Li X
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
FAU - Xu, Zhangye
AU  - Xu Z
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Lv, Jieqiang
AU  - Lv J
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Ge, Ren-Shan
AU  - Ge RS
AUID- ORCID: 0000-0002-3448-9823
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital and 
      Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
LA  - eng
GR  - 81730042/NSFC (RSG)/
PT  - Journal Article
DEP - 20220406
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (Fluorocarbons)
RN  - 0 (perfluorotetradecanoic acid)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Fluorocarbons/adverse effects
MH  - *Leydig Cells
MH  - Male
MH  - *Oxidative Stress
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testis
MH  - Testosterone/blood
OTO - NOTNLM
OT  - Leydig cells
OT  - PFTeDA
OT  - apoptosis
OT  - oxidative stress
OT  - testosterone
EDAT- 2022/04/07 06:00
MHDA- 2022/06/10 06:00
CRDT- 2022/04/06 12:17
PHST- 2022/02/26 00:00 [revised]
PHST- 2022/01/02 00:00 [received]
PHST- 2022/03/19 00:00 [accepted]
PHST- 2022/04/07 06:00 [pubmed]
PHST- 2022/06/10 06:00 [medline]
PHST- 2022/04/06 12:17 [entrez]
AID - 10.1002/tox.23526 [doi]
PST - ppublish
SO  - Environ Toxicol. 2022 Jul;37(7):1790-1802. doi: 10.1002/tox.23526. Epub 2022 Apr 
      6.