PMID- 35386706
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220531
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - The Distributional Characteristics of M2 Macrophages in the Placental Chorionic 
      Villi are Altered Among the Term Pregnant Women With Uncontrolled Type 2 Diabetes 
      Mellitus.
PG  - 837391
LID - 10.3389/fimmu.2022.837391 [doi]
LID - 837391
AB  - AIM: No definite conclusions have been drawn regarding how prolonged exposure to 
      hyperglycemia affects the distribution of macrophages in the placenta, especially 
      in pregnant women with uncontrolled type 2 diabetes mellitus (T2DM). Herein, we 
      explored the distributional characteristics of placental M2 macrophages, 
      including hofbauer cells (HBCs) in the chorionic villi and decidual macrophages, 
      in pregnant women with uncontrolled T2DM. METHODS: Six healthy singleton 
      pregnancies and five uncontrolled T2DM singleton pregnancies were collected. 
      Multicolor immunofluorescence and immunohistochemistry were performed to record 
      M1 macrophages by CD80 and CD86, the general M2 macrophages by CD163, M2a 
      macrophages by CD163 and DG-SIGN, M2b macrophages by CD163 and CD86, and M2c 
      macrophages by CD163 and CD206. Meanwhile, the monocyte marker of CD14 and the 
      general macrophage marker of CD68 were also documented on placenta. RESULTS: In 
      the chorionic villi and decidua, the most common infiltrated macrophages was the 
      general M2. There were only few M1 and M2b macrophages distributed in the 
      placenta of both the healthy and uncontrolled T2DM groups. The infiltrated degree 
      of M2c macrophages was moderate in chorionic villi and decidua. The uncontrolled 
      T2DM and healthy pregnant women had a comparable amount of M2c macrophages 
      infiltration in the chorionic villi (p = 0.158). Notedly, in both of the healthy 
      and uncontrolled T2DM pregnant women, the predominant subtype of M2 macrophages 
      in the chorionic villi was M2a, where it mainly infiltrated around vessels and 
      syncytiotrophoblasts. The uncontrolled T2DM pregnant women had more M2a 
      macrophage infiltration than the healthy pregnant women (p = 0.016). The M2a 
      macrophages in the decidua of the uncontrolled T2DM group were similar to those 
      of the normal group (p = 0.800). Meanwhile, it was in the chorionic villi but not 
      the decidua, that the CD68(+) macrophages and CD14(+) M2a macrophages were also 
      elevated in the uncontrolled T2DM group (p = 0.035 and 0.044, respectively). 
      CONCLUSION: These results confirmed that the M2 macrophages exhibited increased 
      in the chorionic villi of pregnant women with uncontrolled T2DM. The subsets of 
      M2 macrophages in the placental decidua were similar between uncontrolled T2DM 
      pregnant women and normal groups. It may provide a basis for exploring the 
      functions of different subsets of macrophages in the placental chorionic villi.
CI  - Copyright (c) 2022 Zhang, Cui and Yang.
FAU - Zhang, Muqiu
AU  - Zhang M
AD  - Department of Obstetrics and Gynaecology, Peking University First Hospital, 
      Beijing, China.
FAU - Cui, Dong
AU  - Cui D
AD  - Department of Obstetrics and Gynaecology, Peking University First Hospital, 
      Beijing, China.
FAU - Yang, Huixia
AU  - Yang H
AD  - Department of Obstetrics and Gynaecology, Peking University First Hospital, 
      Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220321
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - *Chorionic Villi
MH  - *Diabetes Mellitus, Type 2
MH  - Female
MH  - Humans
MH  - Macrophages
MH  - Placenta
MH  - Pregnancy
MH  - Pregnant Women
PMC - PMC8978304
OTO - NOTNLM
OT  - chorionic villi
OT  - decidua
OT  - macrophage
OT  - placenta
OT  - type 2 dabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/08 06:00
MHDA- 2022/04/09 06:00
PMCR- 2022/01/01
CRDT- 2022/04/07 05:16
PHST- 2021/12/21 00:00 [received]
PHST- 2022/02/21 00:00 [accepted]
PHST- 2022/04/07 05:16 [entrez]
PHST- 2022/04/08 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.837391 [doi]
PST - epublish
SO  - Front Immunol. 2022 Mar 21;13:837391. doi: 10.3389/fimmu.2022.837391. eCollection 
      2022.