PMID- 35391571
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220725
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Print)
IS  - 0026-3672 (Linking)
VI  - 189
IP  - 5
DP  - 2022 Apr 7
TI  - Microneedle-based nanoporous gold electrochemical sensor for real-time 
      catecholamine detection.
PG  - 180
LID - 10.1007/s00604-022-05260-2 [doi]
LID - 180
AB  - Dopamine (DA), epinephrine (EP), and norepinephrine (NEP) are the main 
      catecholamine of clinical interest, as they play crucial roles in the regulation 
      of nervous and cardiovascular systems and are involved in some brain behaviors, 
      such as stress, panic, anxiety, and depression. Therefore, there is an urgent 
      need for a reliable sensing device able to provide their continuous monitoring in 
      a minimally invasive manner. In this work, the first highly nanoporous gold 
      (h-nPG) microneedle-based sensor is presented for continuous monitoring of 
      catecholamine in interstitial fluid (ISF). The h-nPG microneedle-based gold 
      electrode was prepared by a simple electrochemical self-templating method that 
      involves two steps, gold electrodeposition and hydrogen bubbling at the electrode 
      surface, realized by sweeping the potential between + 0.8 V and 0 V vs Ag/AgCl 
      for 25 scans in a 10 mM HAuCl(4) solution containing 2.5 M NH(4)Cl, and 
      successively applying a fixed potential of - 2 V vs Ag/AgCl for 60 s. The 
      resulting microneedle-based h-nPG sensor displays an interference-free total 
      catecholamine detection expressed as NEP concentration, with a very low LOD of 
      100 nM, excellent sensitivity and stability, and fast response time (< 4 s). The 
      performance of the h-nPG microneedle array sensor was successively assessed in 
      artificial ISF and in a hydrogel skin model at typical physiological 
      concentrations.
CI  - (c) 2022. The Author(s).
FAU - Tortolini, Cristina
AU  - Tortolini C
AD  - Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le 
      Aldo Moro 5, 00185, Rome, Italy.
FAU - Cass, Anthony E G
AU  - Cass AEG
AD  - Department of Chemistry & Institute of Biomedical Engineering, Imperial College, 
      London, UK.
FAU - Pofi, Riccardo
AU  - Pofi R
AD  - Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
FAU - Lenzi, Andrea
AU  - Lenzi A
AD  - Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
FAU - Antiochia, Riccarda
AU  - Antiochia R
AUID- ORCID: 0000-0001-8990-8506
AD  - Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le 
      Aldo Moro 5, 00185, Rome, Italy. riccarda.antiochia@uniroma1.it.
LA  - eng
PT  - Journal Article
DEP - 20220407
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Catecholamines)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Catecholamines
MH  - Electrodes
MH  - *Gold
MH  - *Nanopores
MH  - Needles
PMC - PMC8989844
OTO - NOTNLM
OT  - Catecholamine
OT  - Dopamine
OT  - Electrochemical sensor
OT  - Epinephrine
OT  - Microneedles
OT  - Nanoporous gold needle
OT  - Norepinephrine
COIS- The authors declare no competing interests.
EDAT- 2022/04/09 06:00
MHDA- 2022/04/12 06:00
PMCR- 2022/04/07
CRDT- 2022/04/08 05:05
PHST- 2021/11/09 00:00 [received]
PHST- 2022/03/04 00:00 [accepted]
PHST- 2022/04/08 05:05 [entrez]
PHST- 2022/04/09 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/04/07 00:00 [pmc-release]
AID - 10.1007/s00604-022-05260-2 [pii]
AID - 5260 [pii]
AID - 10.1007/s00604-022-05260-2 [doi]
PST - epublish
SO  - Mikrochim Acta. 2022 Apr 7;189(5):180. doi: 10.1007/s00604-022-05260-2.