PMID- 35397443 OWN - NLM STAT- MEDLINE DCOM- 20220428 LR - 20231213 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 236 DP - 2022 May 1 TI - Di-n-butyl phthalate promotes monocyte recruitment via miR-137-3p-SP1-MCP-1 pathway. PG - 113491 LID - S0147-6513(22)00331-1 [pii] LID - 10.1016/j.ecoenv.2022.113491 [doi] AB - Since non-covalent bound character and widespread application in numerous products, people are exposed to di-n-butyl phthalate (DBP) at low levels through various ways. Epidemiological studies suggested an association between DBP exposure and atherosclerosis (AS). Still, molecular mechanisms remain unclear. This study aimed to explore the effects of DBP on monocyte recruitment, a key and initial step of AS. EA.hy926 cells were treated with DBP (10(-9)-10(-5) M) or DMSO as control. Chemotaxis assay was applied to investigate THP-1 recruitment. Expression of mRNA /miRNAs and proteins were measured by qRT-PCR and Western blotting, respectively. Levels of monocyte chemotactic protein 1 (MCP-1) in supernatant were detected by ELISA assay. Receptor internalization assay was performed to confirm C-C chemokine receptor type 2 (CCR2) subcellular localization in THP-1 cells and the binding between CCR2 and MCP-1. Dual-luciferase reporter assay was used to analyze the combination between miR-137-3p and specificity protein 1 (SP1), as well as SP1 and MCP-1. Results showed that number of recruited THP-1 cells after EA.hy926 cells treated by DBP was significantly higher than that in the control group due to promoted MCP-1 expression. In addition, expression of MCP-1 was regulated through miR-137-3p-SP1 cascade. Besides, overexpression of miR-137-3p reversed the increased number of recruited THP-1 cells. Our results implied that DBP might promote THP-1 recruitment by targeting miR-137-3p-SP1-MCP-1 in EA.hy926 cells. CI - Copyright (c) 2022. Published by Elsevier Inc. FAU - Kong, Yi AU - Kong Y AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China. FAU - Wen, Yun AU - Wen Y AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China. FAU - Cao, Guofa AU - Cao G AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China. FAU - Xu, Yuan AU - Xu Y AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China. FAU - Zhang, Chengxiang AU - Zhang C AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Safety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, Nanjing Medical University, Nanjing, China. FAU - Tang, Chunhui AU - Tang C AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China. FAU - Zhang, Jingshu AU - Zhang J AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Safety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, Nanjing Medical University, Nanjing, China. FAU - Wang, Yubang AU - Wang Y AD - The Key Laboratory of Modern Toxicology, Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China; The Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China; Safety Assessment and Research Center for Drug, Pesticide and Veterinary Drug of Jiangsu Province, Nanjing Medical University, Nanjing, China. Electronic address: ncsa@njmu.edu.cn. LA - eng PT - Journal Article DEP - 20220406 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 RN - 0 (Chemokine CCL2) RN - 0 (MIRN137 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (Receptors, Chemokine) RN - 0 (Sp1 Transcription Factor) RN - 0 (SP1 protein, human) RN - 2286E5R2KE (Dibutyl Phthalate) SB - IM MH - *Atherosclerosis/metabolism MH - Chemokine CCL2/genetics/metabolism MH - Dibutyl Phthalate/toxicity MH - Humans MH - *MicroRNAs/genetics/metabolism MH - Monocytes MH - Receptors, Chemokine MH - Sp1 Transcription Factor/genetics/metabolism OTO - NOTNLM OT - Atherosclerosis OT - Di-n-butyl phthalate OT - MiR-137-3p OT - Monocyte chemotactic protein 1 OT - Monocyte recruitment EDAT- 2022/04/10 06:00 MHDA- 2022/04/29 06:00 CRDT- 2022/04/09 20:11 PHST- 2021/11/09 00:00 [received] PHST- 2022/03/31 00:00 [revised] PHST- 2022/04/02 00:00 [accepted] PHST- 2022/04/10 06:00 [pubmed] PHST- 2022/04/29 06:00 [medline] PHST- 2022/04/09 20:11 [entrez] AID - S0147-6513(22)00331-1 [pii] AID - 10.1016/j.ecoenv.2022.113491 [doi] PST - ppublish SO - Ecotoxicol Environ Saf. 2022 May 1;236:113491. doi: 10.1016/j.ecoenv.2022.113491. Epub 2022 Apr 6.