PMID- 35399100 OWN - NLM STAT- MEDLINE DCOM- 20220412 LR - 20220531 IS - 1749-799X (Electronic) IS - 1749-799X (Linking) VI - 17 IP - 1 DP - 2022 Apr 10 TI - Long noncoding RNA PVT1 promotes chondrocyte extracellular matrix degradation by acting as a sponge for miR-140 in IL-1beta-stimulated chondrocytes. PG - 218 LID - 10.1186/s13018-022-03114-4 [doi] LID - 218 AB - BACKGROUND: Osteoarthritis (OA) is a common degenerative joint disease, and chondrocyte extracellular matrix (ECM) degradation is one vital pathological feature of OA. Long noncoding RNA (lncRNA), a new kind of gene regulator, plays an important role in pathogenesis of many diseases like OA. Recent studies have confirmed that lncRNA plasmacytoma variant translocation 1 (PVT1) expression was upregulated in OA patients; however, its effect on ECM degradation remained unknown. METHODS: Cartilage tissue samples were obtained from 6 OA patients admitted in Guangdong Second Traditional Chinese Medicine Hospital. Chondrocytes were isolated and cultured from the collected cartilage tissue. Plasmid construction, RNA interference, cell transfection, fluorescence in situ hybridization (FISH), and pull-down assay were carried out during the research. RESULTS: In this study, PVT1 expression was significantly increased in chondrocytes stimulated by interleukin-1beta (IL-1beta). In addition, inhibition of PVT1 significantly downregulated the increased expressions of ADAM metallopeptidase with thrombospondin type 1 motif-5 (ADAMTS-5) and matrix metalloproteinase-13 (MMP-13) induced by IL-1beta. Further investigation revealed that PVT1 was an endogenous sponge RNA, which directly bound to miR-140 and inhibited miR-140 expression. CONCLUSION: To sum up, this study showed that PVT1 promoted expressions of ADAMTS-5 and MMP-13 as a competing endogenous RNA (ceRNA) of miR-140 in OA, which eventually led to aggravation of ECM degradation, thus providing a new and promising strategy for the treatment of OA. CI - (c) 2022. The Author(s). FAU - Yao, Nan AU - Yao N AUID- ORCID: 0000-0002-8610-1552 AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. nanyao2000@126.com. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. nanyao2000@126.com. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. nanyao2000@126.com. FAU - Peng, Sha AU - Peng S AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. FAU - Wu, Huai AU - Wu H AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. FAU - Liu, Wengang AU - Liu W AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. FAU - Cai, Dake AU - Cai D AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. FAU - Huang, Dane AU - Huang D AD - Guangdong Provincial Second Hospital of Traditional Chinese Medicine (Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine), 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - The Fifth Clinical College of Guangzhou University of Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou City, 510095, Guangdong Province, China. LA - eng GR - 2018A030313584/Guangdong Basic and Applied Basic Research Foundation, China/ GR - B2022208/Medical Scientific Research Foundation of Guangdong Province, China/ GR - 20194002/Administration of Traditional Chinese Medicine of Guangdong Province, China/ GR - 20222011/Administration of Traditional Chinese Medicine of Guangdong Province, China/ PT - Journal Article DEP - 20220410 PL - England TA - J Orthop Surg Res JT - Journal of orthopaedic surgery and research JID - 101265112 RN - 0 (Interleukin-1beta) RN - 0 (MicroRNAs) RN - 0 (Mirn140 microRNA, human) RN - 0 (PVT1 long-non-coding RNA, human) RN - 0 (RNA, Long Noncoding) RN - EC 3.4.24.- (ADAMTS5 Protein) RN - EC 3.4.24.- (Matrix Metalloproteinase 13) SB - IM MH - ADAMTS5 Protein MH - Apoptosis MH - Cells, Cultured MH - *Chondrocytes/metabolism MH - *Extracellular Matrix/metabolism MH - Humans MH - In Situ Hybridization, Fluorescence MH - Interleukin-1beta/metabolism/pharmacology MH - Matrix Metalloproteinase 13/genetics/metabolism MH - *MicroRNAs/metabolism MH - *Osteoarthritis/pathology MH - *RNA, Long Noncoding/metabolism PMC - PMC8996637 OTO - NOTNLM OT - Chondrocyte OT - Osteoarthritis OT - ceRNA OT - lncRNA PVT1 OT - miR-140 COIS- The authors declare that they have no conflict of interest. EDAT- 2022/04/12 06:00 MHDA- 2022/04/13 06:00 PMCR- 2022/04/10 CRDT- 2022/04/11 05:06 PHST- 2021/07/11 00:00 [received] PHST- 2022/03/31 00:00 [accepted] PHST- 2022/04/11 05:06 [entrez] PHST- 2022/04/12 06:00 [pubmed] PHST- 2022/04/13 06:00 [medline] PHST- 2022/04/10 00:00 [pmc-release] AID - 10.1186/s13018-022-03114-4 [pii] AID - 3114 [pii] AID - 10.1186/s13018-022-03114-4 [doi] PST - epublish SO - J Orthop Surg Res. 2022 Apr 10;17(1):218. doi: 10.1186/s13018-022-03114-4.