PMID- 3539998
OWN - NLM
STAT- MEDLINE
DCOM- 19870219
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 25
IP  - 1
DP  - 1987 Jan
TI  - Aspergillus fumigatus-specific antibodies in allergic bronchopulmonary 
      aspergillosis and aspergilloma: evidence for a polyclonal antibody response.
PG  - 5-9
AB  - Patients with the Aspergillus-induced diseases allergic bronchopulmonary 
      aspergillosis (ABPA), aspergilloma (fungus ball), and Aspergillus skin 
      test-positive asthma were differentiated immunologically by radioimmunoassay 
      based on their total immunoglobulin E (IgE) and Aspergillus fumigatus-specific 
      IgE levels. In this study, a new, highly sensitive biotin-avidin-linked 
      immunosorbent assay was used to evaluate A. fumigatus-specific antibodies of all 
      immunoglobulin classes. Studied populations included 13 patients with ABPA, 12 
      with aspergilloma, 9 with Aspergillus skin test-positive asthma, and 9 normal 
      individuals without asthma. A. fumigatus-specific antibodies of all classes were 
      elevated in patients with ABPA, variably elevated in those with aspergilloma, and 
      lowest in the other two groups. This assay demonstrated significantly higher 
      specific IgE antibody levels in the ABPA group over those of the other groups, 
      even with 1:1,000 dilutions of the sera. This study demonstrated that ABPA is a 
      disease characterized by a polyclonal antibody response to Aspergillus antigen 
      and not just a response to IgE and IgG antibody classes. The measurement of other 
      antibody classes, particularly IgD and IgA, could enhance the immunodiagnosis of 
      ABPA. The biotin-avidin-linked immunosorbent assay was found to be a highly 
      sensitive assay that can be a clinically useful alternative to radioimmunoassay 
      in the measurement of A. fumigatus-specific antibodies.
FAU - Brummund, W
AU  - Brummund W
FAU - Resnick, A
AU  - Resnick A
FAU - Fink, J N
AU  - Fink JN
FAU - Kurup, V P
AU  - Kurup VP
LA  - eng
GR  - AI19104/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Fungal/*biosynthesis
MH  - Antigens, Fungal/immunology
MH  - Aspergillosis/*immunology
MH  - Aspergillosis, Allergic Bronchopulmonary/*immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin D/biosynthesis
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Immunoglobulin M/biosynthesis
MH  - Lung Diseases, Fungal/*immunology
PMC - PMC265798
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
PMCR- 1987/01/01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PHST- 1987/01/01 00:00 [pmc-release]
AID - 10.1128/jcm.25.1.5-9.1987 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1987 Jan;25(1):5-9. doi: 10.1128/jcm.25.1.5-9.1987.