PMID- 35400271
OWN - NLM
STAT- MEDLINE
DCOM- 20220412
LR  - 20220716
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 4
DP  - 2022 Apr
TI  - CircSEC24A (hsa_circ_0003528) interference suppresses epithelial-mesenchymal 
      transition of hepatocellular carcinoma cells via miR-421/MMP3 axis.
PG  - 9049-9062
LID - 10.1080/21655979.2022.2057761 [doi]
AB  - Accumulating evidence indicates that circular RNAs (circRNAs) function as 
      conclusive modulators in diverse tumors, including in hepatocellular carcinoma 
      (HCC). Nonetheless, knowledge of the latent mechanisms involving circRNAs in HCC 
      development is insufficient. circSEC24A (hsa_circ_0003528) was discovered by 
      microarray analysis of patients with HCC. Binding sites between circSEC24A, 
      miR-421, miR-421 and matrix metalloproteinase 3 (MMP3) were predicted using 
      online bioinformatics tools. Interactions involving miRNA and target genes or 
      circRNAs were verified by luciferase reporter-gene and RNA pull-down assays. Two 
      HCC cell lines (HCCLM3 and Hep3B) and normal THLE-2 liver cells were used for in 
      vitro experiments. miRNA and mRNA expression levels were detected by RT-qPCR, and 
      protein expression was measured by western blotting. Cell proliferation was 
      evaluated using Cell Counting Kit 8 (CCK-8) assays along with colony formation 
      assays. Cell invasion and migration were determined using the Transwell and wound 
      healing migration assays. A xenograft model was used to evaluate the role of 
      circSEC24A in vivo. circSEC24A expression was significantly upregulated in HCCLM3 
      and Hep3B cells. Silencing circSEC24A mitigated the proliferation, migration, 
      invasion, and epithelial-mesenchymal transition (EMT) of HCC cells, which was 
      abrogated by downregulation of miR-421. Meanwhile, MMP3 could bind to miR-421 to 
      decrease the functional effects of miR-421 and induce tumor metastasis. Knockdown 
      of cicSEC24A suppressed tumor growth in vivo. circSEC24A interference suppressed 
      HCC cell EMT by sponging miR-421, further regulating MMP3, and inhibiting tumor 
      growth in vivo. Therefore, circSEC24A could represent a potential target for HCC 
      patient treatment.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Department of Basic Medicine, Chongqing Medical and Pharmaceutical College, 
      Chongqing, China.
FAU - Zhou, Jian
AU  - Zhou J
AD  - Department of Pathology, The Affiliated Hospital of Southwest Medical University, 
      Luzhou, Sichuan, China.
AD  - Department of Pathology, the First Affiliated Hospital of ChengDu Medical 
      College, Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MIRN421 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - *Carcinoma, Hepatocellular/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - *Liver Neoplasms/metabolism
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
PMC - PMC9161912
OTO - NOTNLM
OT  - MMP3
OT  - circSEC24A
OT  - competing endogenous RNA
OT  - hepatocellular carcinoma (HCC)
OT  - hsa_circ_0003528
OT  - miR-421
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/04/12 06:00
MHDA- 2022/04/13 06:00
PMCR- 2022/04/10
CRDT- 2022/04/11 05:23
PHST- 2022/04/11 05:23 [entrez]
PHST- 2022/04/12 06:00 [pubmed]
PHST- 2022/04/13 06:00 [medline]
PHST- 2022/04/10 00:00 [pmc-release]
AID - 2057761 [pii]
AID - 10.1080/21655979.2022.2057761 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Apr;13(4):9049-9062. doi: 10.1080/21655979.2022.2057761.