PMID- 35400474
OWN - NLM
STAT- MEDLINE
DCOM- 20220412
LR  - 20220525
IS  - 1873-7145 (Electronic)
IS  - 0963-9969 (Linking)
VI  - 155
DP  - 2022 May
TI  - Screening and identification of high bioavailable oligopeptides from rapeseed 
      napin (Brassica napus) protein-derived hydrolysates via Caco-2/HepG2 co-culture 
      model.
PG  - 111101
LID - S0963-9969(22)00158-2 [pii]
LID - 10.1016/j.foodres.2022.111101 [doi]
AB  - Rapeseed napin (Brassica napus) protein-derived hydrolysates (RNPHs, 1-4) are 
      mixtures of peptides, prior to reaching liver tissue and playing their 
      antidiabetic role, at least being absorbed and metabolized by the intestinal 
      barrier. The study aims at screening and identifying high bioavailable rapessed 
      napin-derived oligopeptides via simulated gastrointestinal digestion and 
      absorption. Specifically, RNPHs were obtained using a novel ultrasound-assisted 
      digestive device. The potential capacity of treating type 2 diabetes mellitus 
      (T2DM) was evaluated preliminarily via enhancing glucose transporter 4 (GLUT4) 
      expression and translocation. Also, absorbable rapeseed napin-derived 
      oligopeptides were screened and identified in a Caco-2/HepG2 co-culture model 
      using liquid chromatography coupled with electrospray ionisation and quadrupole 
      time of flight tandem mass spectrometry (LC-ESI-QTOF-MS). The results involved 
      mainly two aspects. First, absorbable oligopeptides from RNPH-1 (Molecular 
      weight, Mw </= 3 kDa) with the highest degree of hydrolysis (DH) were the optimal 
      ones to enhance GLUT4 expression and translocation (P < 0.05). Secondly, 
      oligopeptides (Thr-His-Leu-Pro-Lys (THLPK), His-Leu-Pro-Lys (HLPK), (Ile) 
      Leu-Pro-Lys ((I)LPK), His-Leu-Lys (HLK), and Leu-His-Lys (LHK)), identified from 
      both RNPH-1 and RNPH-2 which significantly enhanced GLUT4 expression and 
      translocation, could be absorbed intact and reached HepG2 cells. These findings 
      indicated that high bioavailable oligopeptides from RNPHs were the potential 
      usefulness to treat T2DM in vitro.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Yao, Meng
AU  - Yao M
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China.
FAU - Yao, Yijun
AU  - Yao Y
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China.
FAU - Qin, Bowen
AU  - Qin B
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China.
FAU - Pan, Mengmeng
AU  - Pan M
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China.
FAU - Ju, Xingrong
AU  - Ju X
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China.
FAU - Xu, Feiran
AU  - Xu F
AD  - Engineering Research Center of Bio-process, Ministry of Education, School of Food 
      and Biological Engineering, Hefei University of Technology, Hefei 230009, 
      People's Republic of China. Electronic address: feiranxu@hfut.edu.cn.
FAU - Wang, Lifeng
AU  - Wang L
AD  - College of Food Science and Engineering/Collaborative Innovation Center for 
      Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, 
      Nanjing 210023, Jiangsu, China. Electronic address: wanglifeng_8@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220306
PL  - Canada
TA  - Food Res Int
JT  - Food research international (Ottawa, Ont.)
JID - 9210143
RN  - 0 (Oligopeptides)
RN  - 0 (Protein Hydrolysates)
SB  - IM
MH  - *Brassica napus
MH  - *Brassica rapa
MH  - Caco-2 Cells
MH  - Coculture Techniques
MH  - *Diabetes Mellitus, Type 2
MH  - Humans
MH  - Oligopeptides/chemistry
MH  - Protein Hydrolysates/chemistry
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - A novel digestive device
OT  - Absorbable compositions
OT  - Antidiabete
OT  - GLUT4 expression and translocation
OT  - In vitro
OT  - LC-ESI-QTOF-MS
EDAT- 2022/04/12 06:00
MHDA- 2022/04/13 06:00
CRDT- 2022/04/11 05:24
PHST- 2022/01/07 00:00 [received]
PHST- 2022/02/02 00:00 [revised]
PHST- 2022/03/01 00:00 [accepted]
PHST- 2022/04/11 05:24 [entrez]
PHST- 2022/04/12 06:00 [pubmed]
PHST- 2022/04/13 06:00 [medline]
AID - S0963-9969(22)00158-2 [pii]
AID - 10.1016/j.foodres.2022.111101 [doi]
PST - ppublish
SO  - Food Res Int. 2022 May;155:111101. doi: 10.1016/j.foodres.2022.111101. Epub 2022 
      Mar 6.