PMID- 35401695
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 13
DP  - 2022
TI  - Effects of Luteinizing Hormone Releasing Hormone A2 on Gonad Development in 
      Juvenile Amur Sturgeon, Acipenser schrenckii, Revealed by Transcriptome Profiling 
      Analysis.
PG  - 859965
LID - 10.3389/fgene.2022.859965 [doi]
LID - 859965
AB  - Acipenser schrenckii is an economically important aquatic species whose gonads 
      require particularly long times to reach sexual maturity. Luteinizing hormone 
      plays important roles in gonad development, and luteinizing hormone releasing 
      hormone A2 (LH-A2) is used as an oxytocin to promote ovulation in aquaculture of 
      A. schrenckii. In this study, we aimed to determine the effects of LH-A2 on gonad 
      development in juvenile A. schrenckii through transcriptome profiling analysis of 
      the pituitary and gonads after LH-A2 treatment at a dose of 3 mug/kg. The 
      17beta-estradiol (E2) levels gradually increased with LH-A2 treatment time, and 
      significantly differed from those of the control group on days 5 and 7 (p < 
      0.01). However, the content of testosterone (Testo) gradually decreased with 
      LH-A2 treatment time and showed significant differences on day 3 (p < 0.05), and 
      on days 5 and 7 (p < 0.01), compared to those in the control group. Thus, LH-A2 
      promotes the secretion of E2 and inhibits the secretion of Testo. Transcriptome 
      profiling analysis revealed a total of 2,883 and 8,476 differentially expressed 
      genes (DEGs) in the pituitary and gonads, respectively, thus indicating that 
      LH-A2 has more regulatory effects on the gonads than the pituitary in A. 
      schrenckii. Signal transduction, global and overview maps, immune system, 
      endocrine system and lipid metabolism were the main enriched metabolic pathways 
      in both the pituitary and gonads. Sixteen important genes were selected from 
      these metabolic pathways. Seven genes were co-DEGs enriched in both signal 
      transduction and endocrine system metabolic pathways. The other co-DEGs were 
      selected from the immune system and lipid metabolism metabolic pathways, and 
      showed mRNA expression changes of >7.0. The expression of five DEGs throughout 
      LH-A2 treatment was verified to show the same patterns of change as those 
      observed with RNA-seq, indicating the accuracy of the RNA-seq in this study. Our 
      findings provide valuable evidence of the regulation of gonad development of 
      juvenile A. schrenckii by LH-A2 and may enable the establishment of artificial 
      techniques to regulate gonad development in this species.
CI  - Copyright (c) 2022 Lv, Jin, Cao, Wang, Jin and Zhang.
FAU - Lv, Weihua
AU  - Lv W
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
FAU - Jin, Shubo
AU  - Jin S
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
FAU - Cao, Dingchen
AU  - Cao D
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
FAU - Wang, Nianmin
AU  - Wang N
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
FAU - Jin, Xing
AU  - Jin X
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of 
      Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20220324
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC8989137
OTO - NOTNLM
OT  - Acipenser schrenckii
OT  - LH-A2
OT  - gonad
OT  - pituitary
OT  - transcriptome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/12 06:00
MHDA- 2022/04/12 06:01
PMCR- 2022/03/24
CRDT- 2022/04/11 05:28
PHST- 2022/01/22 00:00 [received]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/04/11 05:28 [entrez]
PHST- 2022/04/12 06:00 [pubmed]
PHST- 2022/04/12 06:01 [medline]
PHST- 2022/03/24 00:00 [pmc-release]
AID - 859965 [pii]
AID - 10.3389/fgene.2022.859965 [doi]
PST - epublish
SO  - Front Genet. 2022 Mar 24;13:859965. doi: 10.3389/fgene.2022.859965. eCollection 
      2022.