PMID- 35402191
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220413
IS  - 2223-4691 (Print)
IS  - 2223-4691 (Electronic)
IS  - 2223-4683 (Linking)
VI  - 11
IP  - 3
DP  - 2022 Mar
TI  - Effect of Huaji Jianpi Decoction on the semen quality of high-fat diet-induced 
      obese mice.
PG  - 336-347
LID - 10.21037/tau-22-115 [doi]
AB  - BACKGROUND: At present, the incidence of obesity is increasing. Several studies 
      have shown that obesity can reduce male fertility by affecting spermatogenesis 
      and semen quality. Traditional Chinese medicine (TCM) has fewer side effects and 
      stable efficacy in the treatment of related diseases. This study aimed to 
      investigate the effect of Huaji Jianpi Decoction on the semen quality of high-fat 
      diet-induced obese mice. METHODS: The obese male mice model was constructed by 
      using high-fat diet and the Huaji Jianpi Decoction was processed into an aqueous 
      extract. Mice were allocated in the normal group (n=30) and five different 
      treatment groups (n=50). Huaji Jianpi Decoction was applied in low-, medium- and 
      high-dose [17.52 g/(kg.d), 35.04 g/(kg.d) and 70.07 g/(kg.d), respectively]. The 
      body weight, body fat, testis wet weight, testis coefficient, and routine sperm 
      parameters were detected and analyzed. Meanwhile, transmission electron 
      microscope (TEM) was used to observe testis ultrastructure. reverse-transcription 
      quantitative PCR (RT-qPCR) was used to measure the expression of tumour necrosis 
      factor alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1). RESULTS: 
      Compared with normal mice (ND), the testis wet weight and testis coefficient of 
      mice in the blank group were significantly decreased, while the number of 
      mitochondria was observed to be decreased on testis ultrastructure examination, 
      and apoptotic cells and germ cells in the spermatogenic tubules were shed. After 
      Huaji Jianpi Decoction administration, the body fat and blood lipid levels of 
      obese mice were decreased, the testis wet weight and testis coefficient were 
      increased, and semen parameters were increased. Different doses of Huaji Jianpi 
      Decoction could improve testicular weight, sperm density, sperm motility, forward 
      motility, and total sperm motility. Huaji Jianpi Decoction could also 
      downregulate TNF-alpha and MCP-1 expression and inhibit germ cell apoptosis to 
      improve semen quality. CONCLUSIONS: Huaji Jianpi Decoction can improve the semen 
      quality of high-fat diet-induced obese mice by reducing weight and lipid levels.
CI  - 2022 Translational Andrology and Urology. All rights reserved.
FAU - Wu, Yinling
AU  - Wu Y
AD  - Hebei University Health Science Center, Hebei University, Baoding, China.
FAU - Zhang, Miao
AU  - Zhang M
AD  - Hebei University Health Science Center, Hebei University, Baoding, China.
FAU - Zhao, Qinglei
AU  - Zhao Q
AD  - Hebei University Health Science Center, Hebei University, Baoding, China.
FAU - Chen, Lanyu
AU  - Chen L
AD  - NHC Key Laboratory of Family Planning and Healthy, Hebei Key Laboratory of 
      Reproductive Medicine, Hebei Research Institute for Family Planning Science and 
      Technology, Shijiazhuang, China.
FAU - Wang, Guifang
AU  - Wang G
AD  - Hebei University Health Science Center, Hebei University, Baoding, China.
FAU - Ge, Shaoqin
AU  - Ge S
AD  - Hebei University Health Science Center, Hebei University, Baoding, China.
FAU - Wang, Shusong
AU  - Wang S
AD  - NHC Key Laboratory of Family Planning and Healthy, Hebei Key Laboratory of 
      Reproductive Medicine, Hebei Research Institute for Family Planning Science and 
      Technology, Shijiazhuang, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Transl Androl Urol
JT  - Translational andrology and urology
JID - 101581119
PMC - PMC8984972
OTO - NOTNLM
OT  - Huaji Jianpi Decoction
OT  - monocyte chemoattractant protein-1 (MCP-1)
OT  - obese mice
OT  - semen quality
OT  - tumour necrosis factor alpha (TNF-alpha)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://tau.amegroups.com/article/view/10.21037/tau-22-115/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2022/04/12 06:00
MHDA- 2022/04/12 06:01
PMCR- 2022/03/01
CRDT- 2022/04/11 05:30
PHST- 2022/01/17 00:00 [received]
PHST- 2022/03/14 00:00 [accepted]
PHST- 2022/04/11 05:30 [entrez]
PHST- 2022/04/12 06:00 [pubmed]
PHST- 2022/04/12 06:01 [medline]
PHST- 2022/03/01 00:00 [pmc-release]
AID - tau-11-03-336 [pii]
AID - 10.21037/tau-22-115 [doi]
PST - ppublish
SO  - Transl Androl Urol. 2022 Mar;11(3):336-347. doi: 10.21037/tau-22-115.