PMID- 35402638
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221003
IS  - 2329-423X (Print)
IS  - 2329-4248 (Electronic)
IS  - 2329-423X (Linking)
VI  - 9
IP  - 2
DP  - 2022 Apr
TI  - Advances in studying whole mouse brain vasculature using high-resolution 3D light 
      microscopy imaging.
PG  - 021902
LID - 10.1117/1.NPh.9.2.021902 [doi]
LID - 021902
AB  - Significance: The cerebrovasculature has become increasingly recognized as a 
      major player in overall brain health and many brain disorders. Although there 
      have been several landmark studies to understand details of these crucially 
      important structures in an anatomically defined area, brain-wide examination of 
      the whole cerebrovasculature, including microvessels, has been challenging. 
      However, emerging techniques, including tissue processing and three-dimensional 
      (3D) microscopy imaging, enable neuroscientists to examine the total vasculature 
      in the entire mouse brain. Aim: Here, we aim to highlight advances in these 
      high-resolution 3D mapping methods including block-face imaging and light sheet 
      fluorescent microscopy. Approach: We summarize latest mapping tools to understand 
      detailed anatomical arrangement of the cerebrovascular network and the organizing 
      principles of the neurovascular unit (NVU) as a whole. Results: We discuss 
      biological insights gained from studies using these imaging methods and how these 
      tools can be used to advance our understanding of the cerebrovascular network and 
      related cell types in the entire brain. Conclusions: This review article will 
      help to understand recent advance in high-resolution NVU mapping in mice and 
      provide perspective on future studies.
CI  - (c) 2022 The Authors.
FAU - Bennett, Hannah C
AU  - Bennett HC
AD  - The Pennsylvania State University, Department of Neural and Behavioral Sciences, 
      Hershey, Pennsylvania, United States.
FAU - Kim, Yongsoo
AU  - Kim Y
AUID- ORCID: 0000-0002-4277-0279
AD  - The Pennsylvania State University, Department of Neural and Behavioral Sciences, 
      Hershey, Pennsylvania, United States.
LA  - eng
GR  - R01 MH116176/MH/NIMH NIH HHS/United States
GR  - R01 NS108407/NS/NINDS NIH HHS/United States
GR  - RF1 MH124605/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Review
DEP - 20220405
PL  - United States
TA  - Neurophotonics
JT  - Neurophotonics
JID - 101632875
PMC - PMC8983067
OTO - NOTNLM
OT  - block-face imaging
OT  - brain
OT  - cerebrovasculature
OT  - light sheet fluorescence microscopy
OT  - neurovascular unit
OT  - three-dimensional light microscopy
EDAT- 2022/04/12 06:00
MHDA- 2022/04/12 06:01
PMCR- 2022/04/05
CRDT- 2022/04/11 05:32
PHST- 2021/11/30 00:00 [received]
PHST- 2022/03/11 00:00 [accepted]
PHST- 2022/04/11 05:32 [entrez]
PHST- 2022/04/12 06:00 [pubmed]
PHST- 2022/04/12 06:01 [medline]
PHST- 2022/04/05 00:00 [pmc-release]
AID - 21071SSVR [pii]
AID - 10.1117/1.NPh.9.2.021902 [doi]
PST - ppublish
SO  - Neurophotonics. 2022 Apr;9(2):021902. doi: 10.1117/1.NPh.9.2.021902. Epub 2022 
      Apr 5.