PMID- 35409050
OWN - NLM
STAT- MEDLINE
DCOM- 20220413
LR  - 20220416
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 7
DP  - 2022 Mar 28
TI  - The Antiviral Effect of Isatis Root Polysaccharide against NADC30-like PRRSV by 
      Transcriptome and Proteome Analysis.
LID - 10.3390/ijms23073688 [doi]
LID - 3688
AB  - (1) Background: In recent years, the porcine reproductive and respiratory 
      syndrome virus (PRRSV) has become a virulent pathogen that has caused devastating 
      diseases and economic losses worldwide in the swine industry. IRPS has attracted 
      extensive attention in the field of virology. However, it is not clear that IRPS 
      has an antiviral effect on PRRSV at gene and protein levels. (2) Methods: We used 
      transcriptomic and proteomic analysis to investigate the antiviral effect of IRPS 
      against PRRSV. Additionally, a microbiome was used to explore the effects of IRPS 
      on gut microbes. (3) Results: IRPS significantly extenuated the pulmonary 
      pathological lesions and inflammatory response. We used transcriptomic and 
      proteomic analysis to investigate the antiviral effect of IRPS against PRRSV. In 
      the porcine model, 1669 differentially expressed genes (DEGs) and 370 
      differentially expressed proteins (DEPs) were identified. Analysis of the 
      DEG/DEP-related pathways indicated immune-system and infectious-disease (viral) 
      pathways, such as the NOD-like receptor (NLR) signaling pathway, toll-like 
      receptor (TLR) signaling pathway, and Influenza A-associated signaling pathways. 
      It is noteworthy that IRPS can inhibit NLR-dependent gene expression, then reduce 
      the inflammatory damage. IRPS could exert beneficial effects on the host by 
      regulating the structure of intestinal flora. (4) Conclusions: The antiviral 
      effect of IRPS on PRRSV can be directly achieved by omics techniques. 
      Specifically, the antiviral mechanism of IPRS can be better elucidated by 
      screening target genes and proteins using transcriptome and proteome sequencing, 
      and then performing enrichment and classification according to DEGs and DEPs.
FAU - Jiang, Dike
AU  - Jiang D
AUID- ORCID: 0000-0003-1820-3685
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Zhang, Ling
AU  - Zhang L
AD  - College of Veterinary Medicine, Jilin University, Changchun 130012, China.
FAU - Zhu, Guangheng
AU  - Zhu G
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Zhang, Pengfei
AU  - Zhang P
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Wu, Xulong
AU  - Wu X
AD  - Branch of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, 
      Chengdu 611130, China.
FAU - Yao, Xueping
AU  - Yao X
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Luo, Yan
AU  - Luo Y
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Yang, Zexiao
AU  - Yang Z
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Ren, Meishen
AU  - Ren M
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
FAU - Wang, Xinping
AU  - Wang X
AD  - College of Veterinary Medicine, Jilin University, Changchun 130012, China.
FAU - Chen, Sheng
AU  - Chen S
AUID- ORCID: 0000-0003-3526-7808
AD  - Department of Infectious Diseases and Public Health, Jockey Club College of 
      Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong 
      999077, China.
FAU - Wang, Yin
AU  - Wang Y
AD  - Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College 
      of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
LA  - eng
GR  - 2021YFSY0005/the Sichuan Province Science and Technology Planning Project/
PT  - Journal Article
DEP - 20220328
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antiviral Agents)
RN  - 0 (Polysaccharides)
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Antiviral Agents
MH  - *Isatis
MH  - Polysaccharides
MH  - *Porcine Reproductive and Respiratory Syndrome/drug therapy/genetics
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Proteome
MH  - Proteomics
MH  - Swine
MH  - Transcriptome
PMC - PMC8998840
OTO - NOTNLM
OT  - NADC30-like porcine reproductive and respiratory syndrome virus
OT  - isatis root polysaccharide
OT  - transcriptomic and proteomic analysis
COIS- The authors declare that they are no conflict of interest.
EDAT- 2022/04/13 06:00
MHDA- 2022/04/14 06:00
PMCR- 2022/03/28
CRDT- 2022/04/12 01:12
PHST- 2022/02/26 00:00 [received]
PHST- 2022/03/24 00:00 [revised]
PHST- 2022/03/25 00:00 [accepted]
PHST- 2022/04/12 01:12 [entrez]
PHST- 2022/04/13 06:00 [pubmed]
PHST- 2022/04/14 06:00 [medline]
PHST- 2022/03/28 00:00 [pmc-release]
AID - ijms23073688 [pii]
AID - ijms-23-03688 [pii]
AID - 10.3390/ijms23073688 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Mar 28;23(7):3688. doi: 10.3390/ijms23073688.