PMID- 35415819
OWN - NLM
STAT- MEDLINE
DCOM- 20230207
LR  - 20230207
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 201
IP  - 3
DP  - 2023 Mar
TI  - The Palmitoylation/Depalmitoylation Cycle is Involved in the Inhibition of AMPA 
      Receptor Trafficking Induced by Aluminum In Vitro.
PG  - 1398-1406
LID - 10.1007/s12011-022-03234-2 [doi]
AB  - To study the effect of the palmitoylation/depalmitoylation cycle on the 
      inhibition of a-amino-3-hydroxy-5-methyl-4-isoxazolpropionic acid (AMPA) receptor 
      trafficking induced by aluminum (Al) in vitro. Five different doses of 
      aluminum-maltolate complex (Al(mal)(3)) were administered to rat adrenal 
      pheochromocytoma cells (PC12 cells) for three exposure time durations, and the 
      cell activity was measured by the CCK-8 method to obtain the optimal doses and 
      time of Al(mal)(3) exposure. Following Al(mal)(3) exposure, membrane protein (M) 
      and total protein (T) were extracted. The expression levels of GluR1 and GluR2, 
      which are AMPA receptor subunits, were determined by Western blot analysis, and 
      the levels with respect to membrane and total protein were calculated. The ratio 
      of membrane protein to total protein (M/T) was used to measure the rate of AMPA 
      receptor transport. The palmitoylation levels of GluR1 and GluR2 were detected by 
      immunoprecipitation-acyl-biotin exchange (IP-ABE) assay. Western blotting was 
      performed to detect the protein expression of acyltransferase (zDHHC3) and 
      palmitoyl protein thioesterase 1 (PPT1). Following depalmitoylation inhibitor 
      (palmostatin B) treatment of PC12 cells, the effect of aluminum on AMPA receptor 
      trafficking was detected through the aforementioned methods. With increasing 
      Al(mal)(3) doses administered to PC12 cells, a gradual decrease in the 
      trafficking of AMPA receptor subunits GluR1 and GluR2 and in the palmitoylation 
      levels of GluR1 and GluR2 was found; the expression of zDHHC3 was decreased; and 
      the expression of PPT1 was increased. In addition, palmostatin B reduced the 
      effects of Al(mal)(3) on AMPA receptor palmitoylation and trafficking. Al can 
      inhibit the trafficking of the AMPA receptor in vitro, and a decrease in the 
      palmitoylation level of the AMPA receptor may be a mechanism of Al action. The 
      palmitoylation/depalmitoylation cycle of the AMPA receptor is influenced by Al 
      through the actions of zDHHC3 and PPT1.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Song, Jing
AU  - Song J
AUID- ORCID: 0000-0002-0088-821X
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China. sj4933749@126.com.
AD  - Key Lab of Environmental Hazard and Health of Shanxi Province, Shanxi Medical 
      University, Taiyuan, China. sj4933749@126.com.
FAU - Gao, Ting
AU  - Gao T
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China.
FAU - Li, Wenjing
AU  - Li W
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China.
FAU - Yuan, Chunman
AU  - Yuan C
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China.
FAU - Hao, Jiarui
AU  - Hao J
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China.
FAU - Xia, Xinyu
AU  - Xia X
AD  - Department of Occupational Health, School of Public Health, Shanxi Medical 
      University, Taiyuan, China.
LA  - eng
GR  - 2021-083/Research Project Supported by Shanxi Scholarship Council of China/
PT  - Journal Article
DEP - 20220413
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Receptors, AMPA)
RN  - CPD4NFA903 (Aluminum)
RN  - 0 (palmostatin B)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Receptors, AMPA/metabolism
MH  - *Aluminum/pharmacology/metabolism
MH  - Lipoylation/physiology
MH  - Membrane Proteins/metabolism
OTO - NOTNLM
OT  - AMPA receptor trafficking
OT  - Aluminum
OT  - PPT1
OT  - Palmitoylation/depalmitoylation cycle
OT  - zDHHC3
EDAT- 2022/04/14 06:00
MHDA- 2023/02/08 06:00
CRDT- 2022/04/13 05:38
PHST- 2022/03/15 00:00 [received]
PHST- 2022/04/04 00:00 [accepted]
PHST- 2022/04/14 06:00 [pubmed]
PHST- 2023/02/08 06:00 [medline]
PHST- 2022/04/13 05:38 [entrez]
AID - 10.1007/s12011-022-03234-2 [pii]
AID - 10.1007/s12011-022-03234-2 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2023 Mar;201(3):1398-1406. doi: 10.1007/s12011-022-03234-2. 
      Epub 2022 Apr 13.