PMID- 35415912
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220714
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 54
IP  - 7
DP  - 2022 Aug
TI  - High DNA stainability (HDS) should not be recommended as a marker for the 
      detection of sperm DNA damage.
PG  - e14442
LID - 10.1111/and.14442 [doi]
AB  - There was a marker of high DNA stainability (HDS) in the detection of sperm DNA 
      damage, which was defined as the sperm with high green stainability (HIGRN). The 
      sperm with normal double-stranded DNA was stained green by acridine orange (AO). 
      However, the sperm with high green fluorescence or HDS were thought of as 
      immature sperm or the sperm with poor chromatin condensation. Some previous 
      literature reported that the proportion of sperm with HDS increased with age, and 
      had a certain correlation with the poor outcome of assisted reproductive 
      technology. Recently, several articles reported that the marker of HDS decreased 
      linearly with age, which was obviously inconsistent with that reported by the 
      previous literature. In this case, what kind of marker is HDS? Is it worth 
      studying? After extensively reading the literature related to HDS and flow 
      cytometry related books and performing a series of studies related to the 
      detection of sperm DNA damage, we believe that the establishment of HDS in the 
      detection of sperm DNA damage has no theoretical basis and also no support of 
      evidence-based medicine and that using HDS as a marker in the detection of sperm 
      DNA integrity is inappropriate.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Lu, Jin-Chun
AU  - Lu JC
AD  - Center for Reproductive Medicine, Zhongda Hospital, Southeast University, 
      Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20220412
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
RN  - 0 (Biomarkers)
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Biomarkers
MH  - Chromatin
MH  - DNA
MH  - DNA Damage
MH  - DNA Fragmentation
MH  - Humans
MH  - *Infertility, Male/diagnosis/genetics
MH  - Male
MH  - *Semen
MH  - Spermatozoa
OTO - NOTNLM
OT  - flow cytometry
OT  - high DNA stainability
OT  - sperm DNA damage
OT  - sperm chromatin structure analysis
EDAT- 2022/04/14 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/04/13 05:39
PHST- 2022/02/06 00:00 [revised]
PHST- 2021/12/26 00:00 [received]
PHST- 2022/03/30 00:00 [accepted]
PHST- 2022/04/14 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
PHST- 2022/04/13 05:39 [entrez]
AID - 10.1111/and.14442 [doi]
PST - ppublish
SO  - Andrologia. 2022 Aug;54(7):e14442. doi: 10.1111/and.14442. Epub 2022 Apr 12.