PMID- 35415927
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220714
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 54
IP  - 7
DP  - 2022 Aug
TI  - A study of diabetes-induced erectile dysfunction treated with human umbilical 
      cord mesenchymal stem cells.
PG  - e14440
LID - 10.1111/and.14440 [doi]
AB  - The present study aimed to assess the value of human umbilical cord mesenchymal 
      stem cells (hUC-MSCs) for the treatment of diabetes-induced erectile dysfunction 
      (DMED). We established a type 1 diabetes model through intra-abdominal 
      streptozotocin injection. After 10 weeks, an apomorphine test was performed to 
      screen the rats for erectile dysfunction (ED). The rats were divided into three 
      groups: normal control group (n = 10), DMED group (n = 9) and DMED+hUC-MSC group 
      (n = 9). After 4 weeks of hUC-MSC therapy, erectile function was evaluated by 
      intracavernous pressure measurements, and penile tissue collagen and smooth 
      muscle were examined by haematoxylin-eosin and Masson's trichrome staining. In 
      addition, western blotting, immunohistochemistry and RT-PCR analysis of TLR4, 
      VEGF and eNOS were performed. The results showed that hUC-MSC treatment restored 
      erectile function (p < .05) and reversed the smooth muscle/collagen ratio changes 
      of DMED rats (p < .05). Furthermore, hUC-MSC treatment inhibited the expression 
      of TLR4 (p < .05) and enhanced VEGF and eNOS expression (p < .05). In conclusion, 
      hUC-MSC treatment restored the erectile function of diabetic rats by inhibiting 
      TLR4, improving corpora cavernosa fibrosis, and increasing VEGF and eNOS 
      expression.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Wang, Shangren
AU  - Wang S
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Zhang, Aiqiao
AU  - Zhang A
AD  - Department of Neonatology, First Teaching Hospital of Tianjin University of 
      Traditional Chinese Medicine, Tianjin, China.
AD  - Department of Neonatology, National Clinical Research Center for Chinese Medicine 
      Acupuncture and Moxibustion, Tianjin, China.
FAU - Liu, Kang
AU  - Liu K
AUID- ORCID: 0000-0001-7929-6534
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Pan, Yang
AU  - Pan Y
AUID- ORCID: 0000-0003-3962-4278
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Kang, Jiaqi
AU  - Kang J
AUID- ORCID: 0000-0003-4191-854X
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Niu, Shuai
AU  - Niu S
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Song, Yuxuan
AU  - Song Y
AUID- ORCID: 0000-0001-8326-0948
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Zhang, Zhexin
AU  - Zhang Z
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Li, Yuezheng
AU  - Li Y
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Liu, Li
AU  - Liu L
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
FAU - Liu, Xiaoqiang
AU  - Liu X
AUID- ORCID: 0000-0003-3524-6783
AD  - Department of Urology, Tianjin Medical University General Hospital, Tianjin, 
      China.
LA  - eng
GR  - National Natural Science Funds of China (82171594)/
GR  - Zhao Yi-Cheng Medical Science Foundation (ZYYFY2018031)/
PT  - Journal Article
DEP - 20220412
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Experimental/complications/metabolism/therapy
MH  - *Erectile Dysfunction/etiology/metabolism/therapy
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 4
MH  - Umbilical Cord/metabolism
MH  - Vascular Endothelial Growth Factor A
OTO - NOTNLM
OT  - diabetes
OT  - erectile dysfunction
OT  - human umbilical cord mesenchymal stem cell
EDAT- 2022/04/14 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/04/13 05:39
PHST- 2022/03/22 00:00 [revised]
PHST- 2021/12/22 00:00 [received]
PHST- 2022/04/03 00:00 [accepted]
PHST- 2022/04/14 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
PHST- 2022/04/13 05:39 [entrez]
AID - 10.1111/and.14440 [doi]
PST - ppublish
SO  - Andrologia. 2022 Aug;54(7):e14440. doi: 10.1111/and.14440. Epub 2022 Apr 12.