PMID- 35417269
OWN - NLM
STAT- MEDLINE
DCOM- 20220517
LR  - 20220616
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 322
IP  - 5
DP  - 2022 May 1
TI  - The regulating pathway of creatine on muscular protein metabolism depends on the 
      energy state.
PG  - C1022-C1035
LID - 10.1152/ajpcell.00447.2021 [doi]
AB  - Creatine (Cr) is beneficial for increasing muscle mass and preventing muscle 
      atrophy via involving in energy metabolism through the Cr and phosphocreatine 
      (PCr) system. This study aimed to evaluate the supplemental effect of Cr on 
      protein metabolism under normal and starvation conditions. The primary myoblasts 
      were obtained from the breast muscle of chicks. The mammalian target of rapamycin 
      (mTOR)/P70S6 kinase (P70S6K), ubiquitin-proteasome (UP) pathways, and 
      mitochondrial function of myotubes were evaluated at normal or starvation state 
      and with or without glucose supplementation. Under normal condition, Cr 
      supplementation enhanced protein synthesis rate as well as upregulated the total 
      and phosphorylated P70S6K expressions. Cr had little influence on protein 
      catabolism and mitochondrial function. In a starvation state, however, Cr 
      alleviated myotube atrophy and enhanced protein accretion by inhibiting Atrogin1 
      and myostatin (MSTN) expression. Furthermore, Cr treatment upregulated the 
      transcriptional coactivators peroxisome proliferator-activated receptor-gamma 
      coactivator-1alpha (PGC-1alpha) expression and decreased reactive oxygen species (ROS) 
      accumulation under starvation condition. In the presence of glucose, however, the 
      favorable effect of Cr on protein content and myotube diameter did not occur 
      under starvation condition. The present result indicates that at a normal state, 
      Cr stimulated protein synthesis via the mTOR/P70S6K pathway. In a starvation 
      state, Cr mainly takes a favorable effect on protein accumulation via suppression 
      of the UP pathway and mediated mitochondrial function mainly by serving as an 
      energy supplier. The result highlights the potential clinical application for the 
      modulation of muscle mass under different nutritional conditions.
FAU - Sun, Mingfa
AU  - Sun M
AD  - Department of Animal Science, Shandong Provincial Key Laboratory of Animal 
      Biotechnology and Disease Control and Prevention, Shandong Agricultural 
      University, Taian, People's Republic of China.
FAU - Jiao, Hongchao
AU  - Jiao H
AD  - Department of Animal Science, Shandong Provincial Key Laboratory of Animal 
      Biotechnology and Disease Control and Prevention, Shandong Agricultural 
      University, Taian, People's Republic of China.
FAU - Wang, Xiaojuan
AU  - Wang X
AD  - Department of Animal Science, Shandong Provincial Key Laboratory of Animal 
      Biotechnology and Disease Control and Prevention, Shandong Agricultural 
      University, Taian, People's Republic of China.
FAU - Li, Haifang
AU  - Li H
AD  - College of Life Sciences, Shandong Agricultural University, Taian, People's 
      Republic of China.
FAU - Zhou, Yunlei
AU  - Zhou Y
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian, People's Republic of China.
FAU - Zhao, Jingpeng
AU  - Zhao J
AD  - Department of Animal Science, Shandong Provincial Key Laboratory of Animal 
      Biotechnology and Disease Control and Prevention, Shandong Agricultural 
      University, Taian, People's Republic of China.
FAU - Lin, Hai
AU  - Lin H
AUID- ORCID: 0000-0002-0878-137X
AD  - Department of Animal Science, Shandong Provincial Key Laboratory of Animal 
      Biotechnology and Disease Control and Prevention, Shandong Agricultural 
      University, Taian, People's Republic of China.
LA  - eng
SI  - figshare/10.6084/m9.figshare.19235691
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220413
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - MU72812GK0 (Creatine)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Creatine/metabolism/therapeutic use
MH  - Energy Metabolism
MH  - Glucose/metabolism
MH  - Muscle, Skeletal/metabolism
MH  - Muscular Atrophy/metabolism
MH  - *Muscular Diseases/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 
      1-alpha/genetics/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
OTO - NOTNLM
OT  - creatine
OT  - glucose
OT  - mitochondrial quality
OT  - myotubes
OT  - protein metabolism
EDAT- 2022/04/14 06:00
MHDA- 2022/05/18 06:00
CRDT- 2022/04/13 17:11
PHST- 2022/04/14 06:00 [pubmed]
PHST- 2022/05/18 06:00 [medline]
PHST- 2022/04/13 17:11 [entrez]
AID - 10.1152/ajpcell.00447.2021 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2022 May 1;322(5):C1022-C1035. doi: 
      10.1152/ajpcell.00447.2021. Epub 2022 Apr 13.