PMID- 35417768
OWN - NLM
STAT- MEDLINE
DCOM- 20220614
LR  - 20220929
IS  - 0737-0806 (Print)
IS  - 0737-0806 (Linking)
VI  - 114
DP  - 2022 Jul
TI  - Effect of Transvaginal Aspiration of Oocytes on Blood And Peritoneal Fluid 
      Parameters in Mares.
PG  - 103949
LID - S0737-0806(22)00087-9 [pii]
LID - 10.1016/j.jevs.2022.103949 [doi]
AB  - Transvaginal aspiration of oocytes (TVA) is a commonly used clinical procedure to 
      obtain oocytes for in vitro production of embryos in horses. The goal of this 
      study was to evaluate the effect of the TVA procedure on blood and peritoneal 
      parameters, and to investigate the association of these findings with variables 
      such as use of antibiotics, number of ovarian punctures, and length of the 
      procedure. Physical examination was performed and blood and peritoneal fluid were 
      obtained from 14 mares before they underwent TVA and the same parameters were 
      assessed 24 hours after the procedure. On examination, 13/14 mares remained 
      clinically healthy after the procedure. One mare developed low-grade fever, 
      transient anorexia and mild colic that resolved within 12 hours post-TVA. The use 
      of antibiotics, length of procedure and number of ovarian punctures did not have 
      an effect on the measured parameters. All the mares presented significant changes 
      in the leukogram, but these mostly remained within normal reference range values. 
      The peritoneal parameters were also consistently affected after TVA. A 
      significant post-TVA increase in lactate, total protein, and peritoneal 
      neutrophil count was observed in peritoneal fluid. Peritoneal lactate level was 
      elevated above baseline physiological levels in more than 50% of the mares. 
      Results from this study indicate that there is an expected degree of inflammation 
      after TVA procedures and peritoneal fluid parameters could be successfully used 
      to monitor inflammation in the early stages.
CI  - Published by Elsevier Inc.
FAU - Orellana-Guerrero, Daniela
AU  - Orellana-Guerrero D
AD  - William R. Pritchard Veterinary Medical Teaching Hospital, Davis, CA, USA.
FAU - Dini, Pouya
AU  - Dini P
AD  - Departments of Population Health and Reproduction and.
FAU - Santos, Eduardo
AU  - Santos E
AD  - William R. Pritchard Veterinary Medical Teaching Hospital, Davis, CA, USA.
FAU - de la Fuente, Alejandro
AU  - de la Fuente A
AD  - Anatomy, Physiology, and Cell Biology, University of California, Davis, CA, USA.
FAU - Meyers, Stuart
AU  - Meyers S
AD  - Anatomy, Physiology, and Cell Biology, University of California, Davis, CA, USA.
FAU - Koshak, Sara
AU  - Koshak S
AD  - William R. Pritchard Veterinary Medical Teaching Hospital, Davis, CA, USA.
FAU - Dujovne, Ghislaine
AU  - Dujovne G
AD  - Departments of Population Health and Reproduction and. Electronic address: 
      gdujovne@ucdavis.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220410
PL  - United States
TA  - J Equine Vet Sci
JT  - Journal of equine veterinary science
JID - 8216840
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Lactates)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Ascitic Fluid
MH  - Female
MH  - *Horse Diseases
MH  - Horses
MH  - Inflammation/veterinary
MH  - Lactates
MH  - Oocytes
MH  - *Ovarian Follicle/physiology
OTO - NOTNLM
OT  - Equine
OT  - IVP
OT  - OPU
OT  - Oocyte aspiration
OT  - TVA
OT  - Transvaginal aspiration oocytes
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/04/14 06:00
MHDA- 2022/06/15 06:00
CRDT- 2022/04/13 20:06
PHST- 2021/10/15 00:00 [received]
PHST- 2022/04/04 00:00 [revised]
PHST- 2022/04/05 00:00 [accepted]
PHST- 2022/04/14 06:00 [pubmed]
PHST- 2022/06/15 06:00 [medline]
PHST- 2022/04/13 20:06 [entrez]
AID - S0737-0806(22)00087-9 [pii]
AID - 10.1016/j.jevs.2022.103949 [doi]
PST - ppublish
SO  - J Equine Vet Sci. 2022 Jul;114:103949. doi: 10.1016/j.jevs.2022.103949. Epub 2022 
      Apr 10.