PMID- 35418711
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 0003-2719 (Print)
IS  - 1532-236X (Electronic)
IS  - 0003-2719 (Linking)
VI  - 55
IP  - 9
DP  - 2022
TI  - Development and Validation of an Analytical Method to Quantitate Hydroxycitric 
      Acid, the Key Constituent in Garcinia cambogia Extract, in Rodent Plasma and 
      Fetus.
PG  - 1382-1397
LID - 10.1080/00032719.2021.2005618 [doi]
AB  - Garcinia cambogia extract (GCE) is a popular botanical supplement used in weight 
      loss products. Hydroxycitric acid (HCA) is the principal component in GCE. Due to 
      lack of adequate toxicity data to assess the safe use of GCE, the National 
      Toxicology Program is testing GCE in Hsd:Sprague Dawley(R) SD(R) rats following 
      perinatal exposure and in adult B6C3F1/N mice. We report a validated method 
      utilizing sample clean up with ultrafiltration followed by liquid 
      chromatography-tandem mass spectrometry analysis to quantify HCA in rat plasma 
      over the concentration range of 20 to 800 ng/mL. The method was linear (r(2) >/= 
      0.99) with the limits of quantitation (LOQ) and detection (LOD) of 20.0 and 3.9 
      ng/mL plasma, respectively. The accuracy (determined as relative error, RE) and 
      precision (determined as relative standard deviation, RSD) using Quality Control 
      standards analyzed over multiple days were </= +/- 7.5% and </= 9.5%, respectively. The 
      method can be applied to quantify HCA in study matrices (RE </= +/- 23.0%; RSD </= 6.0) 
      except gestational day (GD)18 fetus. The method was partially validated in GD18 
      fetal homogenate over the concentration range 60-3000 ng/g (r(2) >/= 0.99, RE </= +/- 
      11.9%, and RSD </= 5.5%; LOQ 60.0 ng/g; LOD 7.77 ng/g). The standards as high as 
      20,000 ng/mL (plasma) and 502,000 ng/g (fetus) can successfully be quantified 
      after diluting into the validated range (RE </= +/- 2.6%; RSD </= 5.2%). These data 
      demonstrate that the method is suitable to quantify HCA in rodent matrices and 
      can be adapted to other biological matrices.
FAU - Mutlu, Esra
AU  - Mutlu E
AD  - Division of the National Toxicology Program, National Institute of Environmental 
      Health Sciences, Research Triangle Park, NC.
FAU - Pierfelice, Jessica
AU  - Pierfelice J
AD  - Battelle Memorial Institute, Columbus, OH.
FAU - Cao, Yu
AU  - Cao Y
AD  - Battelle Memorial Institute, Columbus, OH.
FAU - Djonabaye, Alison
AU  - Djonabaye A
AD  - Battelle Memorial Institute, Columbus, OH.
FAU - Gleason, Shelly
AU  - Gleason S
AD  - Battelle Memorial Institute, Columbus, OH.
FAU - Burback, Brian
AU  - Burback B
AD  - Battelle Memorial Institute, Columbus, OH.
FAU - Waidyanatha, Suramya
AU  - Waidyanatha S
AD  - Division of the National Toxicology Program, National Institute of Environmental 
      Health Sciences, Research Triangle Park, NC.
LA  - eng
GR  - HHSN273201400027C/ES/NIEHS NIH HHS/United States
GR  - ZIC ES103316/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
DEP - 20211211
PL  - United States
TA  - Anal Lett
JT  - Analytical letters
JID - 0261510
PMC - PMC9004611
MID - NIHMS1765211
OTO - NOTNLM
OT  - Garcinia cambogia extract
OT  - botanical dietary supplements
OT  - hydroxycitric acid
OT  - liquid chromatography-tandem mass spectrometry (LC-MS/MS)
OT  - ultrafiltration clean-up
OT  - validation
COIS- The authors declare no competing financial interest.
EDAT- 2022/04/15 06:00
MHDA- 2022/04/15 06:01
PMCR- 2022/04/12
CRDT- 2022/04/14 05:19
PHST- 2022/04/14 05:19 [entrez]
PHST- 2022/04/15 06:00 [pubmed]
PHST- 2022/04/15 06:01 [medline]
PHST- 2022/04/12 00:00 [pmc-release]
AID - 10.1080/00032719.2021.2005618 [doi]
PST - ppublish
SO  - Anal Lett. 2022;55(9):1382-1397. doi: 10.1080/00032719.2021.2005618. Epub 2021 
      Dec 11.