PMID- 35419461
OWN - NLM
STAT- MEDLINE
DCOM- 20220415
LR  - 20220531
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2022
DP  - 2022
TI  - Long Noncoding RNA Hotair Promotes the Progression and Immune Escape in Laryngeal 
      Squamous Cell Carcinoma through MicroRNA-30a/GRP78/PD-L1 Axis.
PG  - 5141426
LID - 10.1155/2022/5141426 [doi]
LID - 5141426
AB  - Homeobox (HOX) transcript antisense RNA (Hotair) is elevated in many cancers 
      significantly. However, the oncogenic role of Hotair in human laryngeal squamous 
      cell carcinoma (LSCC) is still unknown. Thus, we explored the expression profile 
      of Hotair and its function in LSCC. We observed high expression levels of Hotair 
      in six LSCC cell lines compared to the human nasopharyngeal epithelial cell line. 
      Knockdown of Hotair inhibited proliferation and enhanced apoptosis of Tu212 and 
      Hep-2 cell lines in vitro. Moreover, the overexpression of hsa-miR-30a-5p 
      inhibited the expression of GRP78 and PD-L1, but Hotair overexpression in LSCC 
      cells rescues both proteins. Furthermore, the impacts of hsa-miR-30a-5p 
      upregulation on the apoptosis and proliferation of LSCC cells were rescued by 
      overexpression of Hotair. Finally, we combined si-Hotair and a VEGF inhibitor to 
      treat LSCC cells in vitro or in vivo and surprisingly observed a significant 
      inhibition of LSCC growth. In summary, these results indicate that Hotair 
      displays an oncogenic role in both malignancy and immune escape in LSCC related 
      to hsa-miR-30a-5p/GRP78/PD-L1 signaling. Therefore, Hotair may be a potential 
      target for treating LSCC.
CI  - Copyright (c) 2022 Xiaowei Yuan et al.
FAU - Yuan, Xiaowei
AU  - Yuan X
AD  - Department of Otorhinolaryngology, People's Hospital of Dongxihu District, Wuhan, 
      China.
FAU - Shen, Qinhua
AU  - Shen Q
AD  - Department of Pharmacy, People's Hospital of Dongxihu District, Wuhan, China.
FAU - Ma, Wenxue
AU  - Ma W
AUID- ORCID: 0000-0002-5807-8957
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Second People's Hospital 
      of Jingmen, 39 Xiangshan Road, Jingmen Hubei Province 448000, China.
LA  - eng
PT  - Journal Article
DEP - 20220404
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (MIRN30a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - B7-H1 Antigen/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics/immunology
MH  - MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics/immunology
MH  - *Tumor Escape
PMC - PMC9001128
COIS- None of the authors has any competing interests in the manuscript.
EDAT- 2022/04/15 06:00
MHDA- 2022/04/16 06:00
PMCR- 2022/04/04
CRDT- 2022/04/14 05:22
PHST- 2021/08/16 00:00 [received]
PHST- 2021/12/29 00:00 [revised]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/04/14 05:22 [entrez]
PHST- 2022/04/15 06:00 [pubmed]
PHST- 2022/04/16 06:00 [medline]
PHST- 2022/04/04 00:00 [pmc-release]
AID - 10.1155/2022/5141426 [doi]
PST - epublish
SO  - J Immunol Res. 2022 Apr 4;2022:5141426. doi: 10.1155/2022/5141426. eCollection 
      2022.