PMID- 35421107 OWN - NLM STAT- MEDLINE DCOM- 20220418 LR - 20220514 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 17 IP - 4 DP - 2022 TI - Isolation and optimization of extracellular PHB depolymerase producer Aeromonas caviae Kuk1-(34) for sustainable solid waste management of biodegradable polymers. PG - e0264207 LID - 10.1371/journal.pone.0264207 [doi] LID - e0264207 AB - Bioplastics, synthesized by several microbes, accumulates inside cells under stress conditions as a storage material. Several microbial enzymes play a crucial role in their degradation. This research was carried to test the biodegradability of poly-beta-hydroxybutyrate (PHB) utilizing PHB depolymerase, produced by bacteria isolated from sewage waste soil samples. Potent PHB degrader was screened based on the highest zone of hydrolysis followed by PHB depolymerase activity. Soil burial method was employed to check their degradation ability at different incubation periods of 15, 30, and 45 days at 37+/-2 degrees C, pH 7.0 at 60% moisture with 1% microbial inoculum of Aeromonas caviae Kuk1-(34) (MN414252). Without optimized conditions, 85.76% of the total weight of the PHB film was degraded after 45 days. This degradation was confirmed with Fourier-transform infrared spectroscopy (FTIR) and Scanning electron microscope (SEM) analysis. The presence of bacterial colonies on the surface of the degraded film, along with crest, holes, surface erosion, and roughness, were visible. Media optimization was carried out in statistical mode using Plackett Burman (PB) and Central Composite Design (CCD) of Response Surface Methodology (RSM) by considering ten different factors. Analysis of Variance (ANOVA), Pareto chart, response surface plots, and F-value of 3.82 implies that the above statistical model was significant. The best production of PHB depolymerase enzyme (14.98 U/mL) was observed when strain Kuk1-(34) was grown in a media containing 0.1% PHB, K2HPO4 (1.6 gm/L) at 27 ℃ for seven days. Exploiting these statistically optimized conditions, the culture was found to be a suitable candidate for the management of solid waste, where 94.4% of the total weight of the PHB film was degraded after 45 days of incubation. FAU - Amir, Mohammad AU - Amir M AD - Protein Research Laboratory, Department of Bioengineering, Integral University, Lucknow, India. FAU - Bano, Naushin AU - Bano N AD - Protein Research Laboratory, Department of Bioengineering, Integral University, Lucknow, India. FAU - Baker, Abu AU - Baker A AD - Protein Research Laboratory, Department of Bioengineering, Integral University, Lucknow, India. FAU - Zia, Qamar AU - Zia Q AD - Health and Basic Science Research Centre, Majmaah University, Majmaah, Saudi Arabia. AD - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, Saudi Arabia. FAU - Banawas, Saeed AU - Banawas S AD - Health and Basic Science Research Centre, Majmaah University, Majmaah, Saudi Arabia. AD - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, Saudi Arabia. AD - Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America. FAU - Zaheer, Mohd Rehan AU - Zaheer MR AD - Department of Science, Gagan College of Management and Technology, Aligarh, India. FAU - Shariq, Mohammad AU - Shariq M AD - Department of Physics, Faculty of Science, Jazan University, Jazan, Saudi Arabia. FAU - Nawaz, Md Sarfaraz AU - Nawaz MS AD - Department of Chemistry, Faculty of Science, Jazan University, Jazan, Saudi Arabia. FAU - Khan, Mohd Farhan AU - Khan MF AD - Department of Science, Gagan College of Management and Technology, Aligarh, India. AD - Nano Solver Lab, Department of Mechanical Engineering, Z. H. College of Engineering & Technology, Aligarh Muslim University, Aligarh, India. FAU - Azad, Z R Azaz Ahmad AU - Azad ZRAA AD - Department of Post-Harvest Engineering and Technology, Aligarh Muslim University, Aligarh, India. FAU - Gupta, Anamika AU - Gupta A AD - Department of Chemistry, Aligarh Muslim University, Aligarh, India. FAU - Iqbal, Danish AU - Iqbal D AUID- ORCID: 0000-0002-3033-4226 AD - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah, Saudi Arabia. FAU - Roohi AU - Roohi AUID- ORCID: 0000-0003-4296-6105 AD - Protein Research Laboratory, Department of Bioengineering, Integral University, Lucknow, India. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220414 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Culture Media) RN - 0 (Hydroxybutyrates) RN - 0 (Polyesters) RN - 0 (Polymers) RN - 0 (Soil) RN - 0 (Solid Waste) RN - EC 3.1.1.- (Carboxylic Ester Hydrolases) SB - IM MH - *Aeromonas caviae/metabolism MH - Bacteria/metabolism MH - Carboxylic Ester Hydrolases/metabolism MH - Culture Media MH - Hydroxybutyrates/metabolism MH - Polyesters/chemistry MH - Polymers MH - Soil MH - Solid Waste MH - *Waste Management PMC - PMC9009665 COIS- The authors have declared that no competing interests exist. EDAT- 2022/04/15 06:00 MHDA- 2022/04/19 06:00 PMCR- 2022/04/14 CRDT- 2022/04/14 17:13 PHST- 2021/08/29 00:00 [received] PHST- 2022/02/05 00:00 [accepted] PHST- 2022/04/14 17:13 [entrez] PHST- 2022/04/15 06:00 [pubmed] PHST- 2022/04/19 06:00 [medline] PHST- 2022/04/14 00:00 [pmc-release] AID - PONE-D-21-27980 [pii] AID - 10.1371/journal.pone.0264207 [doi] PST - epublish SO - PLoS One. 2022 Apr 14;17(4):e0264207. doi: 10.1371/journal.pone.0264207. eCollection 2022.