PMID- 35421926
OWN - NLM
STAT- MEDLINE
DCOM- 20220418
LR  - 20220716
IS  - 1471-2393 (Electronic)
IS  - 1471-2393 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Apr 14
TI  - Factors associated with fetal karyotype in spontaneous abortion: a case-case 
      study.
PG  - 320
LID - 10.1186/s12884-022-04491-8 [doi]
LID - 320
AB  - BACKGROUND: Most embryos that spontaneously abort during early pregnancy are 
      found to have chromosomal abnormalities. The purpose of this study is to explore 
      the factors involved in chromosome aberrations during embryogenesis. METHODS: A 
      case-case study was performed to compare the risk factors for spontaneous 
      abortion with and without embryo chromosome aberration. A total of 160 cases of 
      spontaneous abortion were enrolled from a tertiary general hospital in Kunming. 
      KaryoLite BACs-on-Beads (KL-BoBs) and fluorescence in situ hybridization (FISH) 
      were employed to determine chromosomal constitution of abortion chorion villus 
      samples. Maternal serum levels of homocysteine (Hcy) were detected by high 
      performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). 
      Information about clinical background and environmental exposure was collected 
      through a self-designed questionnaire. To identify the inherited chromosomal 
      abnormalities, couples with chromosomal abnormalities in abortus were recalled 
      for karyotyping. RESULTS: The overall rate of chromosomal abnormalities was 62.5% 
      (100/160, KL-BoBs combined with FISH) including 51.9% (83/160) aneuploidies, 6.3% 
      (10/160) polyploidies, and 4.4% (7/160) structural abnormalities. Only one case 
      of structural abnormality was found to be inherited from maternal balanced 
      translocation. Compared to abortus with normal karyotype, abortus with abnormal 
      karyotype showed a positive association with parental age and elevated maternal 
      serum homocysteine (Hcy) level, but negative association with previous 
      miscarriage and perceived noise. CONCLUSIONS: Embryonic chromosomal aberrations 
      accounted for the majority of spontaneous abortion cases. A combination of 
      internal and external factors may induce spontaneous abortion through fetal 
      chromosomal aberrations or other pathogenic mechanisms.
CI  - (c) 2022. The Author(s).
FAU - Xu, Qinghua
AU  - Xu Q
AD  - School of Public Health, Kunming Medical University, No. 1168, Chunrongxi Road, 
      Yuhua Street, Chenggong, Kunming, 650500, Yunnan, China.
AD  - Kunming Children's Hospital (Children's Hospital Affiliated to Kunming Medical 
      University), No. 288, Qianxing Road, Kunming, 650228, Yunnan, China.
FAU - Chan, Ying
AU  - Chan Y
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Feng, Yun
AU  - Feng Y
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Zhu, Baosheng
AU  - Zhu B
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Yang, Bicheng
AU  - Yang B
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Zhu, Shu
AU  - Zhu S
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Su, Lingyun
AU  - Su L
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Zou, Li
AU  - Zou L
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Feng, Na
AU  - Feng N
AD  - The First People's Hospital of Yunnan Province, No. 157, Jinbi Road, Kunming, 
      650034, Yunnan, China.
FAU - Li, Yan
AU  - Li Y
AUID- ORCID: 0000-0003-0806-7034
AD  - School of Public Health, Kunming Medical University, No. 1168, Chunrongxi Road, 
      Yuhua Street, Chenggong, Kunming, 650500, Yunnan, China. yanli20021965@21cn.com.
LA  - eng
GR  - 81673186/national natural science foundation of china/
GR  - 2015YNPHXT01/yunnan provincial collaborative innovation center for public health 
      and disease prevention and control grant/
PT  - Journal Article
DEP - 20220414
PL  - England
TA  - BMC Pregnancy Childbirth
JT  - BMC pregnancy and childbirth
JID - 100967799
RN  - 0LVT1QZ0BA (Homocysteine)
SB  - IM
MH  - *Abortion, Spontaneous/genetics
MH  - Chromosome Aberrations
MH  - Female
MH  - Homocysteine
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Karyotyping
MH  - Pregnancy
MH  - Tandem Mass Spectrometry
PMC - PMC9012016
OTO - NOTNLM
OT  - Chromosomal abnormality
OT  - Environment
OT  - Genetic factor
OT  - Homocysteine
OT  - Spontaneous abortion
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/04/16 06:00
MHDA- 2022/04/19 06:00
PMCR- 2022/04/14
CRDT- 2022/04/15 05:03
PHST- 2021/10/06 00:00 [received]
PHST- 2022/02/17 00:00 [accepted]
PHST- 2022/04/15 05:03 [entrez]
PHST- 2022/04/16 06:00 [pubmed]
PHST- 2022/04/19 06:00 [medline]
PHST- 2022/04/14 00:00 [pmc-release]
AID - 10.1186/s12884-022-04491-8 [pii]
AID - 4491 [pii]
AID - 10.1186/s12884-022-04491-8 [doi]
PST - epublish
SO  - BMC Pregnancy Childbirth. 2022 Apr 14;22(1):320. doi: 10.1186/s12884-022-04491-8.