PMID- 35423227
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231108
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 11
IP  - 13
DP  - 2021 Feb 10
TI  - Photosensitizer-loaded hydrogels for photodynamic inactivation of multirestistant 
      bacteria in wounds.
PG  - 7600-7609
LID - 10.1039/d0ra09786a [doi]
AB  - Photodynamic treatment is a promising tool for the therapy of multidrug-resistant 
      bacteria. In this study, we highlight photosensitizer-loaded hydrogels as an 
      application system for infected wounds. The poly(ethylene glycol) 
      diacrylate-based and electron beam-polymerized hydrogels were mechanically stable 
      and transparent. They were loaded with two photoactive, porphyrin-based drugs - 
      tetrakis(1 methylpyridinium-4-yl)porphyrin p-toluenesulfonate (TMPyP) and 
      tetrahydroporphyrin - p toluenesulfonate (THPTS). The hydrogels released a 
      sufficient amount of the photosensitizers (up to 300 mumol l(-1)), relevant for 
      efficiency. The antimicrobial effectivity of loaded hydrogels was investigated in 
      a tissue-like system as well as in a liquid system against a multiresistant 
      Escherichia coli. In both systems, light induced eradication was possible. In 
      contrast, hydrogels alone showed only minor antimicrobial activity. Furthermore, 
      the loaded hydrogels were successfully tested against seven multidrug-resistant 
      bacterial strains, namely Enterococcus faecium, Staphylococcus aureus, Klebsiella 
      pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli and 
      Achromobacter xylosoxidans. The eradication of these pathogens, except A. 
      xylosoxidans, was successfully demonstrated. In general, TMPyP-loaded hydrogels 
      were more effective than THPTS-loaded ones. Nevertheless, both photosensitizers 
      displayed effectivity against all investigated bacteria strains. Taken together, 
      our data demonstrate that photosensitizer-loaded hydrogels are a promising new 
      tool to improve the treatment of wounds infected with problematic bacterial 
      pathogens.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Glass, Sarah
AU  - Glass S
AD  - Leibniz Institute of Surface Engineering (IOM) Permoserstrasse 15 D-04318 Leipzig 
      Germany agnes.schulze@iom-leipzig.de.
FAU - Kuhnert, Mathias
AU  - Kuhnert M
AD  - Leibniz Institute of Surface Engineering (IOM) Permoserstrasse 15 D-04318 Leipzig 
      Germany agnes.schulze@iom-leipzig.de.
FAU - Lippmann, Norman
AU  - Lippmann N
AD  - Institute of Medical Microbiology and Epidemiology of Infectious Diseases, 
      University Hospital Leipzig Liebigstrasse 21 D-04103 Leipzig Germany.
FAU - Zimmer, Joanne
AU  - Zimmer J
AUID- ORCID: 0000-0001-7298-4625
AD  - Leibniz Institute of Surface Engineering (IOM) Permoserstrasse 15 D-04318 Leipzig 
      Germany agnes.schulze@iom-leipzig.de.
FAU - Werdehausen, Robert
AU  - Werdehausen R
AD  - Department of Anesthesiology and Intensive Care Medicine, University Hospital 
      Leipzig Liebigstrasse 20 D-04103 Leipzig Germany.
FAU - Abel, Bernd
AU  - Abel B
AUID- ORCID: 0000-0001-6032-1680
AD  - Leibniz Institute of Surface Engineering (IOM) Permoserstrasse 15 D-04318 Leipzig 
      Germany agnes.schulze@iom-leipzig.de.
FAU - Eulenburg, Volker
AU  - Eulenburg V
AUID- ORCID: 0000-0002-4878-5746
AD  - Department of Anesthesiology and Intensive Care Medicine, University Hospital 
      Leipzig Liebigstrasse 20 D-04103 Leipzig Germany.
FAU - Schulze, Agnes
AU  - Schulze A
AUID- ORCID: 0000-0003-1316-1367
AD  - Leibniz Institute of Surface Engineering (IOM) Permoserstrasse 15 D-04318 Leipzig 
      Germany agnes.schulze@iom-leipzig.de.
LA  - eng
PT  - Journal Article
DEP - 20210217
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC8694937
COIS- There are no conflicts to declare.
EDAT- 2021/02/17 00:00
MHDA- 2021/02/17 00:01
PMCR- 2021/02/17
CRDT- 2022/04/15 05:25
PHST- 2020/11/18 00:00 [received]
PHST- 2021/02/10 00:00 [accepted]
PHST- 2022/04/15 05:25 [entrez]
PHST- 2021/02/17 00:00 [pubmed]
PHST- 2021/02/17 00:01 [medline]
PHST- 2021/02/17 00:00 [pmc-release]
AID - d0ra09786a [pii]
AID - 10.1039/d0ra09786a [doi]
PST - epublish
SO  - RSC Adv. 2021 Feb 17;11(13):7600-7609. doi: 10.1039/d0ra09786a. eCollection 2021 
      Feb 10.