PMID- 35423732
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230921
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 11
IP  - 20
DP  - 2021 Mar 23
TI  - A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic 
      nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug.
PG  - 12361-12373
LID - 10.1039/d1ra00939g [doi]
AB  - In this paper, we propose a fluorescent biosensor for the sequential detection of 
      Pb(2+) ions and the cancer drug epirubicin (Epn) using the interactions between 
      label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a 
      metal-phenolic nanomaterial (i.e., nano-monoclinic copper-tannic acid 
      (NMc-CuTA)). An exploration of the sensing mechanism shows that LFGr-ssDNA and AO 
      strongly adsorb on NMc-CuTA through pi-pi stacking and electrostatic interactions, 
      and this results in the fluorescence quenching of AO. In order to sense the 
      target Pb(2+), initially, LFGr-ssDNA specifically binds with Pb(2+) ions to form 
      a G4 complex (G-Pb(2+)-G base pair), which was released from the surface of 
      NMc-CuTA with strong AO fluorescence enhancement (Turn-ON). The subsequent 
      addition of a biothiol, like cysteine (Cys), to the G4 complex decreases the 
      fluorescence, as the Pb(2+) ions released from the G4 complex have a higher 
      interaction affinity with the sulfur atoms of Cys; this further induces the 
      unwinding of the G4 complex to form LFGr-ssDNA. Finally, Epn was added to this, 
      which intercalates with LFGr-ssDNA to form a G4 complex via G-Epn-G, resulting in 
      fluorescence recovery (Turn-ON). Accordingly, the Turn-ON fluorescent probe had 
      subsequent limits of detection of 1.5 and 5.6 nM for Pb(2+) and Epn, 
      respectively. Hence, the reported NMc-CuTA-based sensing platform has potential 
      applications for the detection of Pb(2+) and Epn in real samples with good 
      sensitivity and selectivity.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Arunjegan, A
AU  - Arunjegan A
AD  - Department of Chemistry, SRM Institute of Science and Technology Kattankulathur 
      Tamil Nadu 603 203 India panneerp1@srmist.edu.in panneerchem82@gmail.com +91 
      9688538842.
FAU - Rajaji, P
AU  - Rajaji P
AD  - Department of Chemistry, SRM Institute of Science and Technology Kattankulathur 
      Tamil Nadu 603 203 India panneerp1@srmist.edu.in panneerchem82@gmail.com +91 
      9688538842.
FAU - Sivanesan, S
AU  - Sivanesan S
AUID- ORCID: 0000-0002-2103-4862
AD  - Department of Applied Science and Technology, A. C Technology, Anna University 
      Chennai Tamil Nadu 600 025 India.
FAU - Panneerselvam, P
AU  - Panneerselvam P
AUID- ORCID: 0000-0003-2647-6835
AD  - Department of Chemistry, SRM Institute of Science and Technology Kattankulathur 
      Tamil Nadu 603 203 India panneerp1@srmist.edu.in panneerchem82@gmail.com +91 
      9688538842.
LA  - eng
PT  - Journal Article
DEP - 20210329
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC8696957
COIS- The authors declare no competing financial interests.
EDAT- 2022/04/16 06:00
MHDA- 2022/04/16 06:01
PMCR- 2021/03/29
CRDT- 2022/04/15 05:26
PHST- 2021/02/04 00:00 [received]
PHST- 2021/03/09 00:00 [accepted]
PHST- 2022/04/15 05:26 [entrez]
PHST- 2022/04/16 06:00 [pubmed]
PHST- 2022/04/16 06:01 [medline]
PHST- 2021/03/29 00:00 [pmc-release]
AID - d1ra00939g [pii]
AID - 10.1039/d1ra00939g [doi]
PST - epublish
SO  - RSC Adv. 2021 Mar 29;11(20):12361-12373. doi: 10.1039/d1ra00939g. eCollection 
      2021 Mar 23.