PMID- 35426037
OWN - NLM
STAT- MEDLINE
DCOM- 20230109
LR  - 20230220
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 30
IP  - 1
DP  - 2023 Jan
TI  - Melatonin Increases Proliferation and Decreases Apoptosis of GC-1 spg Cells by 
      Upregulating the Expression of circTec.
PG  - 135-144
LID - 10.1007/s43032-022-00937-8 [doi]
AB  - Melatonin has been shown to be beneficial for the motility of human sperm, 
      although its mechanism remains to be uncovered. Circular RNAs (circRNAs) have 
      been shown to regulate cellular function in many diseases. However, there has 
      been no relevant research on the effect of melatonin on sperm circRNAs. In this 
      study, we aimed to explore the changes in circRNAs after melatonin treatment of 
      GC-1 spg cells and identify key functional circRNAs. The results showed that 
      melatonin enhanced the proliferation and reduced the apoptosis of GC-1 spg cells. 
      A total of 1423 circRNAs were found to be significantly differentially expressed 
      between groups with and without melatonin treatment. Of these circRNAs, 702 were 
      upregulated and 721 were downregulated. circTec was one of the upregulated 
      circRNAs. Suppressing the expression of circTec significantly reduced cell 
      proliferation and mammalian target of rapamycin (mTOR) signaling pathway 
      activation but promoted melatonin-treated GC-1 spg cell apoptosis. In conclusion, 
      melatonin increased the expression of circTec to exert its physiological effects 
      on GC-1 spg cells, possibly by activating the mTOR signaling pathway. These 
      results enhance our understanding of the biological function of circTec and its 
      regulation by melatonin in spermatogenesis and infertility.
CI  - (c) 2022. Society for Reproductive Investigation.
FAU - Xu, Changlong
AU  - Xu C
AD  - Department of Reproductive Medical Center, the Second Nanning People's Hospital, 
      No. 13 Dancun Road, Nanning, 530031, China. jirongmei539828578@163.com.
FAU - Yang, Hua
AU  - Yang H
AD  - Department of Reproductive Medical Center, the Second Nanning People's Hospital, 
      No. 13 Dancun Road, Nanning, 530031, China.
FAU - Li, Chunyuan
AU  - Li C
AD  - Department of Reproductive Medical Center, the Second Nanning People's Hospital, 
      No. 13 Dancun Road, Nanning, 530031, China.
FAU - Wu, Zhuo
AU  - Wu Z
AD  - Department of Reproductive Medical Center, the Second Nanning People's Hospital, 
      No. 13 Dancun Road, Nanning, 530031, China.
FAU - Ma, Yafeng
AU  - Ma Y
AD  - Department of Obstetrics and Gynecology, Wuxiang Hospital of Nanning Second 
      People's Hospital, Nanning, 530031, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220414
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - JL5DK93RCL (Melatonin)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Male
MH  - Apoptosis
MH  - Cell Proliferation
MH  - *Melatonin/pharmacology
MH  - *MicroRNAs/metabolism
MH  - *RNA, Circular
MH  - Semen/metabolism
MH  - TOR Serine-Threonine Kinases
MH  - Animals
MH  - Mice
MH  - Cell Line
OTO - NOTNLM
OT  - Infertility
OT  - Melatonin
OT  - RNA sequencing
OT  - Spermatogenesis
OT  - circTec
EDAT- 2022/04/16 06:00
MHDA- 2023/01/06 06:00
CRDT- 2022/04/15 05:33
PHST- 2021/11/09 00:00 [received]
PHST- 2022/04/02 00:00 [accepted]
PHST- 2022/04/16 06:00 [pubmed]
PHST- 2023/01/06 06:00 [medline]
PHST- 2022/04/15 05:33 [entrez]
AID - 10.1007/s43032-022-00937-8 [pii]
AID - 10.1007/s43032-022-00937-8 [doi]
PST - ppublish
SO  - Reprod Sci. 2023 Jan;30(1):135-144. doi: 10.1007/s43032-022-00937-8. Epub 2022 
      Apr 14.