PMID- 35426182
OWN - NLM
STAT- MEDLINE
DCOM- 20220517
LR  - 20220716
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 36
IP  - 5
DP  - 2022 May
TI  - MALAT1 regulates PCT expression in sepsis patients through the miR-125b/STAT3 
      axis.
PG  - e24428
LID - 10.1002/jcla.24428 [doi]
LID - e24428
AB  - BACKGROUND: Procalcitonin (PCT) is an important marker in diagnosing sepsis. 
      However, some other diseases can also cause an increase in PCT. PCT still has 
      some limitations in the clinical application of diagnosing sepsis. Therefore, it 
      is of great significance to clarify the regulatory mechanism of PCT expression in 
      sepsis and provide new therapeutic targets for sepsis. METHODS: Blood samples 
      from clinical patients were collected, and peripheral blood monocytes were 
      isolated. Bioinformatics was performed to find the ceRNA regulatory network of 
      STAT3/PCT. MALAT1 and miR-125b were detected by qRT-PCR. MALAT1 was located by 
      fluorescence in situ hybridization (FISH) in U937 cells, and the regulatory 
      relationship between MALAT1, miR-125b, and STAT3 was verified by double 
      luciferase activity report and RNA pull-down assay. U937 cells were transfected 
      with miR-125b, and the effects of the MALAT1/miR-125b/STAT3 pathway on gene and 
      protein secretion levels of PCT were verified by qRT-PCR, western blot, and 
      ELISA. RESULTS: In the serum of sepsis patients and 
      lipopolysaccharide(LPS)-induced U937 cells, MALAT1, STAT3, and PCT gene 
      expression levels were significantly increased, while miR-125b expression level 
      was decreased. FISH results showed that the MALAT1 transcript was mainly located 
      in the nucleus. The double luciferase activity report and RNA pull-down assay 
      results suggested a targeted regulatory relationship between MALAT1, miR-125b, 
      and STAT3. LPS-induced U937 cells transfection with MALAT1 siRNA decreased STAT3 
      protein expression and phosphorylation level and the expression of PCT. 
      Co-transfection with miR-125b inhibitor effectively reversed this phenomenon. 
      CONCLUSIONS: MALAT1 could upregulate the expressions of STAT3 and PCT by targeted 
      adsorption of miR-125b.
CI  - (c) 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Le, Yuanjie
AU  - Le Y
AD  - Department of Emergency, Hwamei Hospital, University of Chinese Academy of 
      Sciences, Ningbo, China.
AD  - Ningbo Institute of Life and Health Industry, University of Chinese Academy of 
      Sciences, Ningbo, China.
FAU - Shi, Yongwei
AU  - Shi Y
AUID- ORCID: 0000-0001-5673-217X
AD  - Department of Emergency, Hwamei Hospital, University of Chinese Academy of 
      Sciences, Ningbo, China.
AD  - Ningbo Institute of Life and Health Industry, University of Chinese Academy of 
      Sciences, Ningbo, China.
LA  - eng
GR  - 2022-F16/Ningbo Key Support Medical Discipline/
GR  - 2020ky839/Medical health Science and Technology project of Zhejiang Province/
PT  - Journal Article
DEP - 20220415
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (MIRN125 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Procalcitonin)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lipopolysaccharides
MH  - *MicroRNAs/genetics
MH  - *Procalcitonin/metabolism
MH  - *RNA, Long Noncoding/genetics
MH  - STAT3 Transcription Factor/genetics/metabolism
MH  - *Sepsis/genetics
PMC - PMC9102486
OTO - NOTNLM
OT  - MALAT1
OT  - STAT3
OT  - miR-125b
OT  - procalcitonin
OT  - sepsis
COIS- The authors declare that they have no competing interests.
EDAT- 2022/04/16 06:00
MHDA- 2022/05/18 06:00
PMCR- 2022/04/15
CRDT- 2022/04/15 05:34
PHST- 2022/02/24 00:00 [revised]
PHST- 2021/12/17 00:00 [received]
PHST- 2022/03/02 00:00 [accepted]
PHST- 2022/04/16 06:00 [pubmed]
PHST- 2022/05/18 06:00 [medline]
PHST- 2022/04/15 05:34 [entrez]
PHST- 2022/04/15 00:00 [pmc-release]
AID - JCLA24428 [pii]
AID - 10.1002/jcla.24428 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2022 May;36(5):e24428. doi: 10.1002/jcla.24428. Epub 2022 Apr 
      15.