PMID- 35427568
OWN - NLM
STAT- MEDLINE
DCOM- 20220526
LR  - 20230916
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 219
DP  - 2022 Jun
TI  - Expression of axon guidance ligands and their receptors in the cornea and 
      trigeminal ganglia and their recovery after corneal epithelium injury.
PG  - 109054
LID - S0014-4835(22)00134-8 [pii]
LID - 10.1016/j.exer.2022.109054 [doi]
AB  - Axon guidance proteins are essential for axonal pathfinding during development. 
      In adulthood, they have been described as pleiotropic proteins with multiple 
      roles in different organs and tissues. While most studies on the roles of these 
      proteins in the cornea have been performed on the Semaphorin family members, with 
      few reports on Netrins or Ephrins, their function in corneal epithelium wound 
      healing and functional nerve regeneration is largely unknown. Here, we studied 
      the expression of ligands belonging to three distinct axon guidance families 
      (Semaphorins, Ephrins, and Netrins) and their most commonly associated receptors 
      in the cornea and trigeminal ganglia (TG) using immunofluorescence staining and 
      RT-qPCR. We also evaluated how their expression recovers after corneal epithelium 
      injury. We found that all ligands studied (Sema3A, Sema3F, EphrinB1, EphrinB2, 
      Netrin-1, and Netrin-4) are abundantly expressed in both the TG and corneal 
      epithelium. Similarly, their receptors (Neuropilin-1, Neuropilin-2, PlexinA1, 
      PlexinA3, EphB2, EphB4, Neogenin, UNC5H1 and DCC) are also expressed in both 
      tissues. Upon corneal epithelium injury, quick recovery of both ligands and 
      receptors was observed at the protein and gene expression levels. While the 
      timing and expression levels vary among these proteins, in general, most of them 
      remained upregulated for several weeks after injury. We propose that the initial 
      protein expression recovery may be related to corneal epithelium recovery since 
      Sema3A, EphrinB2 and Netrin-4 accelerated corneal epithelial cells wound healing. 
      The sustained high expression levels may be functionally related to nerve 
      regeneration and/or patterning. Whilst further studies are required to test this 
      hypothesis, this work contributes to unraveling their function in normal and 
      injured cornea.
CI  - Copyright (c) 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Guaiquil, Victor H
AU  - Guaiquil VH
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, 
      Chicago, IL, USA. Electronic address: vguaiqui@uic.edu.
FAU - Xiao, Cissy
AU  - Xiao C
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, 
      Chicago, IL, USA.
FAU - Lara, Daniel
AU  - Lara D
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, 
      Chicago, IL, USA.
FAU - Dimailig, Greigory
AU  - Dimailig G
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, 
      Chicago, IL, USA.
FAU - Zhou, Qiang
AU  - Zhou Q
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, 
      Chicago, IL, USA.
LA  - eng
GR  - P30 EY001792/EY/NEI NIH HHS/United States
GR  - R01 EY027912/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220412
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Ephrins)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Netrins)
RN  - 0 (SEMA3F protein, human)
RN  - 0 (Semaphorin-3A)
SB  - IM
MH  - Adult
MH  - Axon Guidance
MH  - Cornea/metabolism
MH  - Ephrins/metabolism
MH  - *Epithelium, Corneal/metabolism
MH  - Humans
MH  - Ligands
MH  - Membrane Proteins/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Netrins/metabolism
MH  - Semaphorin-3A/genetics/metabolism
MH  - Trigeminal Ganglion/metabolism
PMC - PMC9133167
MID - NIHMS1800321
OTO - NOTNLM
OT  - Axon guidance proteins
OT  - Cornea wound healing
OT  - Ephrin
OT  - Netrin
OT  - Semaphorin
EDAT- 2022/04/16 06:00
MHDA- 2022/05/27 06:00
PMCR- 2022/06/01
CRDT- 2022/04/15 20:10
PHST- 2021/11/07 00:00 [received]
PHST- 2022/03/15 00:00 [revised]
PHST- 2022/03/22 00:00 [accepted]
PHST- 2022/04/16 06:00 [pubmed]
PHST- 2022/05/27 06:00 [medline]
PHST- 2022/04/15 20:10 [entrez]
PHST- 2022/06/01 00:00 [pmc-release]
AID - S0014-4835(22)00134-8 [pii]
AID - 10.1016/j.exer.2022.109054 [doi]
PST - ppublish
SO  - Exp Eye Res. 2022 Jun;219:109054. doi: 10.1016/j.exer.2022.109054. Epub 2022 Apr 
      12.