PMID- 35428692
OWN - NLM
STAT- MEDLINE
DCOM- 20220519
LR  - 20220530
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 208
IP  - 10
DP  - 2022 May 15
TI  - Berberine Modulates Macrophage Activation by Inducing Glycolysis.
PG  - 2309-2318
LID - 10.4049/jimmunol.2100716 [doi]
AB  - Classical activation of macrophage and monocyte differentiation induced by 
      beta-glucan is accompanied with metabolic change in glucose. However, the role of 
      the metabolic rewiring in monocyte/macrophage activation remains elusive. In this 
      study, we show that berberine induces aerobic glycolysis by blocking the 
      tricarboxylic acid cycle and modulates cytokine responses in bone marrow-derived 
      macrophages (BMDMs) from mice and human PBMC. 13-Methyberberine had activities on 
      glucose metabolism and BMDM activation similar to those of berberine, whereas 
      other tested derivatives lost both activities. Glucose transporter (GLUT)1 
      expression and total cellular hexokinase activity increased gradually in BMDMs in 
      the presence of berberine. In the contrast, LPS upregulated GLUT1 and 
      6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) levels in 6 h. 
      Extracellular glucose levels and replacing glucose with galactose in culture 
      medium affected the cytokine secretion of BMDMs. Berberine alleviated enteritis 
      of Salmonella typhimurium infection and protected mice against endotoxic shock. 
      In mice i.p. injected with LPS, the increase of serum TNF-alpha and the drop of blood 
      glucose were attenuated by berberine treatment. These data together demonstrated 
      that macrophage activation was closely related with glucose metabolism.
CI  - Copyright (c) 2022 by The American Association of Immunologists, Inc.
FAU - Li, Min
AU  - Li M
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Zhang, Haifeng
AU  - Zhang H
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Zhang, Yameng
AU  - Zhang Y
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Fan, Jinting
AU  - Fan J
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Zhu, Jinhao
AU  - Zhu J
AUID- ORCID: 0000-0002-5730-3155
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Gu, Xinyue
AU  - Gu X
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Li, Zhonghui
AU  - Li Z
AUID- ORCID: 0000-0001-8762-7612
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Zhang, Chengyang
AU  - Zhang C
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Liu, Zhen
AU  - Liu Z
AD  - College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 
      China.
FAU - Li, Yuebai
AU  - Li Y
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Zang, Mingxi
AU  - Zang M
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Jin, Ge
AU  - Jin G
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and.
FAU - Li, Guoping
AU  - Li G
AD  - College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 
      China.
FAU - Mi, Yang
AU  - Mi Y
AUID- ORCID: 0000-0002-2585-9917
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Zhengzhou University, Zhengzhou, China; and miyang@zzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220415
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0I8Y3P32UF (Berberine)
RN  - EC 2.7.1.105 (Phosphofructokinase-2)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Berberine/pharmacology
MH  - Glucose
MH  - Glycolysis
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - *Macrophage Activation
MH  - Mice
MH  - Phosphofructokinase-2/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2022/04/17 06:00
MHDA- 2022/05/20 06:00
CRDT- 2022/04/16 05:20
PHST- 2021/07/19 00:00 [received]
PHST- 2022/02/28 00:00 [accepted]
PHST- 2022/04/17 06:00 [pubmed]
PHST- 2022/05/20 06:00 [medline]
PHST- 2022/04/16 05:20 [entrez]
AID - jimmunol.2100716 [pii]
AID - 10.4049/jimmunol.2100716 [doi]
PST - ppublish
SO  - J Immunol. 2022 May 15;208(10):2309-2318. doi: 10.4049/jimmunol.2100716. Epub 
      2022 Apr 15.