PMID- 35428780
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2058-7716 (Print)
IS  - 2058-7716 (Electronic)
IS  - 2058-7716 (Linking)
VI  - 8
IP  - 1
DP  - 2022 Apr 15
TI  - Suppressive effects of umbilical cord mesenchymal stem cell-derived exosomal 
      miR-15a-5p on the progression of cholangiocarcinoma by inhibiting CHEK1 
      expression.
PG  - 205
LID - 10.1038/s41420-022-00932-7 [doi]
LID - 205
AB  - Currently, surgical extraction is the main therapy for cholangiocarcinoma (CCA) 
      patients, but it's highly susceptible to postsurgical complications and 
      recurrence rate. Thus, we identified the suppressing roles of exosomal miR-15a-5p 
      from umbilical cord mesenchymal stem cells (UCMSCs) in the EMT and metastasis of 
      CCA. The microarray dataset GSE265566 was employed to determine the expression of 
      CHEK1 in CCA tissues. The relationship of miR-15a-5p with CHEK1 was analyzed 
      using bioinformatics tools and dual-luciferase reporter assay. The particle size 
      of HUCMSCs-exo was detected by scanning electron microscopy and nanoparticle 
      tracking analysis. The cellular and tumorous phenotypes were assessed through 
      flow cytometry, CCK-8 assay, Transwell assay and the in vivo tumor xenograft 
      experiments. CHEK1 was predicated to be markedly elevated in CCA. miR-15a-5p 
      targeted CHEK1 and downregulated the expression of CHEK1. HUCMSCs-exo activated 
      cell apoptosis but repressed the proliferative, invasive, and migratory 
      potentials of CCA cells. After miR-15a-5p was silenced, HUCMSCs-exo presented an 
      opposite effect in regulating CCA. Overexpression of miR-15a-5p promoted 
      apoptosis but suppressed malignancy and tumorigenicity of CCA cells as well as 
      EMT through downregulating CHEK1. Our data suggested that miR-15a-5p in 
      HUCMSCs-exo suppresses EMT and metastasis of CCA through targeting downregulation 
      of CHEK1.
CI  - (c) 2022. The Author(s).
FAU - Li, Nuo
AU  - Li N
AD  - Department of Gastroenterology, The Fourth Affiliated Hospital of China Medical 
      University, Shenyang, 110000, P.R. China.
FAU - Wang, Baoming
AU  - Wang B
AUID- ORCID: 0000-0002-3154-1665
AD  - Department of Intervention, The Fourth Affiliated Hospital of China Medical 
      University, Shenyang, 110000, P.R. China. wbaoming76@yeah.net.
LA  - eng
PT  - Journal Article
DEP - 20220415
PL  - United States
TA  - Cell Death Discov
JT  - Cell death discovery
JID - 101665035
PMC - PMC9012823
COIS- The authors declare no competing interests.
EDAT- 2022/04/17 06:00
MHDA- 2022/04/17 06:01
PMCR- 2022/04/15
CRDT- 2022/04/16 05:21
PHST- 2021/09/07 00:00 [received]
PHST- 2022/03/02 00:00 [accepted]
PHST- 2022/02/17 00:00 [revised]
PHST- 2022/04/17 06:00 [pubmed]
PHST- 2022/04/17 06:01 [medline]
PHST- 2022/04/16 05:21 [entrez]
PHST- 2022/04/15 00:00 [pmc-release]
AID - 10.1038/s41420-022-00932-7 [pii]
AID - 932 [pii]
AID - 10.1038/s41420-022-00932-7 [doi]
PST - epublish
SO  - Cell Death Discov. 2022 Apr 15;8(1):205. doi: 10.1038/s41420-022-00932-7.